Characterization of Butyrate Uptake by Nontransformed Intestinal Epithelial Cell Lines

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Gonçalves P (Autor) FMUP; Araújo JR (Autor) FMUP; Martel, F (Autor) FMUP

Revista

Título: Journal of Membrane Biology

Vol. 240

Páginas: 35-46

ISSN: 0022-2631

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 31 Citações

Scopus - 38 Citações

Outras Informações

ID Authenticus: P-002-TQ6

DOI: 10.1007/s00232-011-9340-3

Abstract (EN): Butyrate (BT) is one of the main end products of anaerobic bacterial fermentation of dietary fiber within the human colon. Among its recognized effects, BT inhibits colon carcinogenesis. Our aim was to characterize uptake of BT by two nontransformed intestinal epithelial cell lines: rat small intestinal epithelial (IEC-6) and fetal human colonic epithelial (FHC) cells. Uptake of (14)C-BT by IEC-6 cells was (1) time- and concentration-dependent; (2) pH-dependent; (3) Na(+)-, Cl(-)- and energy-dependent; (4) inhibited by BT structural analogues; (5) sensitive to monocarboxylate transporter 1 (MCT1) inhibitors; and (6) insensitive to DIDS and amiloride. IEC-6 cells express both MCT1 and Na(+)-coupled monocarboxylate transporter 1 (SMCT1) mRNA. We conclude that (14)C-BT uptake by IEC-6 cells mainly involves MCT1, with a small contribution of SMCT1. Acute exposure to ethanol, acetaldehyde, indomethacin, resveratrol and quercetin reduced (14)C-BT uptake. Chronic exposure to resveratrol and quercetin reduced (14)C-BT uptake but had no effect on either MCT1 or SMCT1 mRNA levels. Uptake of (14)C-BT by FHC cells was time- and concentration-dependent but pH-, Na(+)-, Cl(-)- and energy-independent and insensitive to BT structural analogues and MCT1 inhibitors. Although MCT1 (but not SMCT1) mRNA expression was found in FHC cells, the characteristics of (14)C-BT uptake by FHC cells did not support either MCT1 or SMCT1 involvement. In conclusion, uptake characteristics of (14)C-BT differ between IEC-6 and FHC cells. IEC-6 cells demonstrate MCT1- and SMCT1-mediated transport, while FHC cells do not.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12