Differences between duodenal and jejunal rat alkaline phosphatase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Conceicao Calhau (Autor) FCNAUP; Martel, F (Autor) FMUP; Hipolito Reis, C (Autor) Outra; Azevedo, I (Autor) Outra

Revista

Título: Clinical Biochemistry

Vol. 33

Páginas: 571-577

ISSN: 0009-9120

Editora: Elsevier

Indexação

ISI Web of Knowledge - 27 Citações

Scopus - 31 Citações

Outras Informações

ID Authenticus: P-000-YT0

DOI: 10.1016/s0009-9120(00)00171-5

Abstract (EN): Objectives: The aim of this study was to kinetically characterize rat tissue-nonspecific-alkaline phosphatase (TNS-ALP) and intestinal (duodenal- and jejunal-IALP), and to determine the effect of substances known to affect phosphorylation/dephosphorylation on TNS- and IALP activity. Design and results: The ranking order of ALP activity (K-enzyme) was duodenal mucosa (IALP) > jejunal mucosa (IALP) > kidney (TNS- LP) > brain (TNS-ALP). Levamisole was found to produce a concentration-dependent decrease of ALP activity in kidney and brain. However, levamisole had no effect on duodenal ALP activity and produced a concentration-dependent increase on jejunum ALP activity. In brain and jejunum homogenates, octreotide, a stable somatostatin analogue, produced a concentration-dependent increase in ALP activity. In relation to duodenum ALP activity, octreotide produced a biphasic effect. Reverse transcription-polymerase chain reaction showed the presence of IALP-I mRNA both in duodenal and jejunal mucosa, but IALP-II only in duodenal mucosa. Conclusions: The results show that duodenal- and jejunal-IALP differ in kinetic parameters and in drug sensitivity. Thus, we can speculate on a different physiologic role for duodenal- and jejunal-IALP, particularly in relation to their dephosphorylation targets. Copyright (C) 2000 The Canadian Society of Clinical Chemists.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7