Folic acid uptake by the human syncytiotrophoblast: Interference by pharmacotherapy, drugs of abuse and pathological conditions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Elisa Keating (Autor) FMUP; Gonçalves P (Autor) FMUP; Campos, I (Autor) Outra; Costa, F (Autor) Outra; Martel, F (Autor) FMUP

Revista

Título: Reproductive Toxicology

Vol. 28

Páginas: 511-520

ISSN: 0890-6238

Editora: Elsevier

Indexação

ISI Web of Knowledge - 37 Citações

Scopus - 39 Citações

Outras Informações

ID Authenticus: P-003-E7C

DOI: 10.1016/j.reprotox.2009.07.001

Abstract (EN): Folic acid (FA) is crucial for fetal development. We aimed to study the modulation of FA placental uptake by: hyperserotoninemia and hyperglycaemia, anti-hypertensives, insulin and drugs of abuse. For this, we characterized (3)H-FA uptake by primary cultures of human cytotrophoblasts (TB cells) and tested the effects of these compounds upon 3H-FA uptake, TB cell viability and gene expression. Our results show that: (a) acutely, 3H-FA uptake was decreased by labetalol (0.1-1000 mu M), ecstasy and amphetamine (0.025-25 mu M); and (b) chronically, (3)H-FA uptake was decreased by high glucose (30 mM), atenolol, nicotine (0.1 and 10 mu M), ethanol (0.01 and 10 mM), ecstasy, amphetamine (0.25 and 1 mu M) and tetrahydrocannabinol (1 and 100 nM). Moreover, many of these drugs were cytotoxic and they differentially modulated the mRNA expression of FA placental transport systems. Our results suggest that inhibition of FA placental uptake may constitute one of the mechanisms involved in the fetortoxicity of many of the compounds tested.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10