Listeria monocytogenes produces a pro-invasive factor that signals via ErbB2/ErbB3 heterodimers

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Oliveira, MJ (Autor) Outra; Lauwaet, T (Autor) Outra; De Bruyne, G (Autor) Outra; Mareel, M (Autor) Outra; Leroy, A (Autor) Outra

Revista

Título: Journal of Cancer Research and Clinical Oncology

Vol. 131

Páginas: 49-59

ISSN: 0171-5216

Editora: Springer Nature

Indexação

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-008-P61

DOI: 10.1007/s00432-004-0601-8

Abstract (EN): Purpose: We have previously demonstrated that conditioned medium from bacteria, some of which were isolated from the colon of cancer patients, stimulate cancer cell invasion in vitro through a 13-mer ß-casein-derived peptide. Since invasion signalling pathways are coordinated by the balance between protein kinases and phosphatases, we investigated the effect of conditioned medium from bacteria on the overall cellular tyrosine phosphorylation. Methods: The tyrosine phosphorylation level of HCT-8/E11 human colon cancer cells treated with the pro-invasive conditioned medium of Listeria, prepared on top of collagen type I gels (CMCollListeria/TSB), were analysed by means of immunoprecipitation and Western blot, with specific anti-phosphotyrosine antibodies. Results: We demonstrated that CM CollListeria/TSB increases the tyrosine phosphorylation level of ErbB2 and ErbB3, members of the epidermal growth factor receptor (EGFR) family, and the association between ErbB3 and the phosphatidylinositol 3-kinase (PI3K) regulatory subunit (p85¿). CMCollListeria/TSB-stimulated ErbB3 tyrosine phosphorylation and cancer cell invasion were independent from EGFR expression and activity but dependent on ErbB2 activity. Conclusions: The interaction between Listeria and collagen type I produces, next to the 13-mer peptide, at least another pro-invasive factor that signals via ErbB2/ErbB3 heterodimers. © Springer-Verlag 2004.

Idioma: Inglês

Tipo (Avaliação Docente): Científica