Three-dimensional scaffolds of fetal decellularized hearts exhibit enhanced potential to support cardiac cells in comparison to the adult

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Silva, AC (Autor) Outra; Rodrigues, SC (Autor) Outra; Caldeira, J (Autor) Outra; Nunes, AM (Autor) Outra; Sampaio Pinto, V (Autor) Outra; Resende, TP (Autor) Outra; Oliveira, MJ (Autor) FMUP; Barbosa, MA (Autor) Outra; Thorsteinsdottir, S (Autor) Outra; Nascimento, DS (Autor) ICBAS; Perpetua Pinto do O (Autor) ICBAS

Revista

Título: Biomaterials

Vol. 104

Páginas: 52-64

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 39 Citações

Scopus - 40 Citações

Outras Informações

ID Authenticus: P-00K-NXG

DOI: 10.1016/j.biomaterials.2016.06.062

Abstract (EN): A main challenge in cardiac tissue engineering is the limited data on microenvironmental cues that sustain survival, proliferation and functional proficiency of cardiac cells. The aim of our study was to evaluate the potential of fetal (E18) and adult myocardial extracellular matrix (ECM) to support cardiac cells. Acellular three-dimensional (3D) bioscaffolds were obtained by parallel decellularization of fetal and adult-heart explants thereby ensuring reliable comparison. Acellular scaffolds retained main constituents of the cardiac ECM including distinctive biochemical and structural meshwork features of the native equivalents. In vitro, fetal and adult ECM-matrices supported 3D culture of heart-derived Sca-1(+) progenitors and of neonatal cardiomyocytes, which migrated toward the center of the scaffold and displayed elongated morphology and excellent viability. At the culture end-point, more Sca-1(+) cells and cardiomyocytes were found adhered and inside fetal bioscaffolds, compared to the adult. Higher repopulation yields of Sca-1(+) cells on fetal ECM relied on beta 1-integrin independent mitogenic signals. Sca-1(+) cells on fetal bioscaffolds showed a gene expression profile that anticipates the synthesis of a permissive microenvironment for cardiomyogenesis. Our findings demonstrate the superior potential of the 3D fetal microenvironment to support and instruct cardiac cells. This knowledge should be integrated in the design of next-generation biomimetic materials for heart repair.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13