Synergistic and antibiofilm properties of ocellatin peptides against multidrug-resistant Pseudomonas aeruginosa

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Bessa, LJ (Autor) Outra; Eaton, P (Autor) Outra; Dematei, A (Autor) Outra; Placido, A (Autor) Outra; Nuno Vale (Autor) ICBAS; Paula Gomes (Autor) FCUP; Delerue Matos, C (Autor) Outra; Leite, JRSA (Autor) Outra; Paula Gameiro (Autor) FCUP

Revista

Título: Future Microbiology

Vol. 13

Páginas: 151-163

ISSN: 1746-0913

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 45 Citações

Scopus - 49 Citações

Outras Informações

ID Authenticus: P-00N-REB

DOI: 10.2217/fmb-2017-0175

Abstract (EN): To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8x the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa. [GRAPHICS] .

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13