Chemical Kinetic Strategies for High-Throughput Screening of Protein Aggregation Modulators

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Zsuzsa Sárkány (Autor) FEUP; Fernando Rocha (Autor) FEUP; Ana M. Damas (Autor) Outra; Sandra Macedo Ribeiro (Autor) Outra; Pedro M. Martins (Autor) Outra

Revista

Título: Chemistry - An Asian Journal

Vol. 14 Nº 4

Páginas: 500-508

ISSN: 1861-4728

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 3 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

LEPABE-2-ECO-INNOVATION - LEPABE-2-ECO-INNOVATION

Outras Informações

ID Authenticus: P-00Q-6GV

DOI: 10.1002/asia.201801703

Abstract (EN): Insoluble aggregates staining positive to amyloid dyes are known histological hallmarks of different neurodegenerative disorders and of type II diabetes. Soluble oligomers are smaller assemblies whose formation prior to or concomitant with amyloid deposition has been associated to the processes of disease propagation and cell death. While the pathogenic mechanisms are complex and differ from disease to disease, both types of aggregates are important biological targets subject to intense investigation in academia and industry. Here we review recent advances in the fundamental understanding of protein aggregation that can be used on the development of anti-amyloid and anti-oligomerization drugs. Specifically, we pinpoint the chemical kinetic aspects that should be attended during the development of high-throughput screening assays and in the hit validation phase. The strategies here devised are expected to establish a connection between basic research and pharmaceutical innovation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9