A mollusk VDR/PXR/CAR-like (NR1J) nuclear receptor provides insight into ancient detoxification mechanisms

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Cruzeiro, C (Autor) Outra; Lopes Marques, M (Autor) Outra; Ruivo, R (Autor) Outra; Rodrigues Oliveira, N (Autor) Outra; Santos, MM (Autor) FCUP; rocha, mj (Autor) ICBAS; rocha, e (Autor) ICBAS; Filipe F C Castro (Autor) FCUP

Revista

Título: Aquatic Toxicology

Vol. 174

Páginas: 61-69

ISSN: 0166-445X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-00K-79C

DOI: 10.1016/j.aquatox.2016.02.007

Abstract (EN): The origin and diversification of the metazoan endocrine systems represents a fundamental research issue in biology. Nuclear receptors are critical components of these systems. A particular group named VDR/PXR/CAR (NR1I/J) is central in the mediation of detoxification responses. While orthologues have been thoroughly characterized in vertebrates, a sparse representation is currently available for invertebrates. Here, we provide the first isolation and characterization of a lophotrochozoan protostome VDR/PXR/CAR nuclear receptor (NR1J), in the estuarine bivalve the peppery furrow shell (Scrobicularia piano). Using a reporter gene assay, we evaluated the xenobiotic receptor plasticity comparing the human PXR with the S. plana NR1J beta. Our results show that the molluscan receptor responds to a natural toxin (okadaic acid) in a similar fashion to that reported for other invertebrates. In contrast, the pesticide esfenvalerate displayed a unique response, since it down regulated transactivation at higher concentrations, while for triclosan no response was observed. Additionally, we uncovered lineage specific gene duplications and gene loss in the gene group encoding NRs in protostomes with likely impacts on the complexity of detoxification mechanisms across different phyla. Our findings pave the way for the development of multi-specific sensor tools to screen xenobiotic compounds acting via the NR1I/J group.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9