Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Rui M. Gil da Costa (Autor) FEUP; Rita Araújo (Autor) Outra; Joana M. O. Santos (Autor) Outra; Mara Fernandes (Autor) Outra; Tiago Neto (Autor) Outra; Hugo Sousa (Autor) Outra; Joana Ribeiro (Autor) Outra; Margarida M. S. M. Bastos (Autor) FEUP; Paula A. Oliveira (Autor) Outra; Diogo Carmo (Autor) Outra; Fátima Casaca (Autor) Outra; Sandra Silva (Autor) Outra; Carlos Lopes (Autor) Outra; Rui Medeiros (Autor) Outra

Revista

Título: Anticancer Research

Vol. 37

Páginas: 2913-2918

ISSN: 0250-7005

Editora: International Institute of Anticancer Research

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00M-TZ1

DOI: 10.21873/anticanres.11644

Abstract (EN): Background/Aim: Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. Materials and Methods: Female transgenic (HPV16(+/-)) and wild-type (HPV16(-/-)) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR. Results: HPV16(+/-) mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16(+/-) animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice. Conclusion: In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6