Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Pereira, FM (Autor) Outra; Bernacka Wojcik, I (Autor) Outra; Ribeiro, RSR (Autor) Outra; Lobato, MT (Autor) Outra; Fortunato, E (Autor) Outra; Martins, R (Autor) Outra; Igreja, R (Autor) Outra; Jorge, PAS (Autor) FCUP; Aguas, H (Autor) Outra; Gonzalez Oliva, AMG (Autor) Outra

Revista

Título: Micromachines

Vol. 7

Página Final: 181

ISSN: 2072-666X

Editora: MDPI

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00M-6PF

DOI: 10.3390/mi7100181

Abstract (EN): This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15