Chromatographic separation of prebiotic oligosaccharides. Case study: Separation of galacto-oligosaccharides on a cation exchanger

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Łukasz Wianiewski (Autor) Outra; Carla S. M. Pereira (Autor) FEUP; Milan Polakovic (Autor) Outra; Alírio E. Rodrigues (Autor) FEUP

Revista

Título: Adsorption

Vol. 20 Nº 2-3

Páginas: 483-492

ISSN: 0929-5607

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 2 Citações

INSPEC

Projetos Associados

LA 20 - PROJECTO ESTRATÉGICO - LA 20 - 2013-2014

Outras Informações

ID Authenticus: P-009-JF2

DOI: 10.1007/s10450-013-9587-3

Abstract (EN): Chromatographic separation of prebiotic oligosaccharides, galacto-oligosaccharides from a typical post-reaction mixture containing unreacted substrate lactose and by-products glucose and galactose was investigated. A commercial cation-exchange resin Dowex 50WX4 was chosen as a prospective preparative-scale adsorbent and separation performance of its four ionic forms, H+, Na+, K+ and Ca2+, was tested. Since adsorption equilibrium isotherms were linear within the entire investigated range of concentrations, they were determined by pulse experiments for all saccharides present in a typical post reaction mixture including tri- and tetragalacto-oligosaccharides. From the four counter ions listed above, hydrogen offered the best selectivity and column performance. The selected H+-form of the cation exchanger was further investigated by means of frontal analysis where high ionic strength and elevated viscosity appear and can play a substantial role. Breakthrough curves were measured for monosaccharides, lactose, glucose-lactose mixture and a multicomponent commercial mixture of galacto-oligosaccharides, Vivinal® GOS. The breakthrough curves were successfully described by the dispersive plug flow model with linear driving force approximation. © 2013 Springer Science+Business Media New York.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10