Study of the suitability of 2-(N-morpholino) ethanesulfonic acid pH buffer for heavy metals accumulation studies using Saccharomyces cerevisiae

• Tipo: Artigo em Revista Científica Internacional
• Data: 2000

Autores

Eduardo V. Soares (Autor) Outra; Andreia P. R. S. Duarte (Autor) Outra; Helena Soares (Autor) FEUP

Revista

Título: Chemical Speciation and Bioavailability

Vol. 12 Nº 2

Páginas: 59-65

ISSN: 0954-2299

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 14 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-001-18Z

DOI: 10.3184/095422900782775535

Abstract (EN): Yeast cells of Saccharomyces cerevisiae maintained viability, and no detectable amount of proteins and inorganic phosphate were determined in the filtrate, when incubated during 48 hours in 10 mM of 2-(N-morpholino) ethanesulfonic acid (MES) at pH 6.0. Ultraviolet light-absorbing materials (at 260 nm) increased between 2 and 6 hours (or 24 hours, depending on the strain) of incubation and decreased after this period. The possibility of using MES pH buffer for in vitro heavy metals accumulation studies is discussed.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7