The attraction of Mac-1(+) phagocytes during acute inflammation by methyl-coated self-assembled monolayers

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Judite N Barbosa (Autor) Outra; Madureira, P (Autor) Outra; Mário Adolfo Barbosa (Autor) FEUP; AP Águas (Autor) ICBAS

Revista

Título: Biomaterials

Vol. 26 Nº 5

Páginas: 3021-3027

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 14 Citações

Outras Informações

ID Authenticus: P-000-2Y2

DOI: 10.1016/j.biomaterials.2004.09.002

Abstract (EN): We have used self-assembled monolayers (SAMs) of alkanethiolates on gold to study the contribution of methyl terminal functional groups in implant-triggered inflammation. The CH3-coated biomaterials were inserted in an air-pouch cavity of the BALB/c mouse and the in situ inflammatory response was monitored 4, 24, 48 and 72 h later. Flow cytometry was applied to define surface expression of the adhesion receptor Mac-1 (CD11b/CD18), a marker of activated leukocytes, and also of CD3 and B220 antigens (T and B lymphocytes). The CH3-coated surfaces caused a significant enhancement in the number of Mac-1(+) cells in the implant. The only significant change in T and B lymphocytes was a transient increase in T cells detected 48 h after the implantation. Peak numbers of Mac-1(+) phagocytes were observed 24 h after implantation. We conclude that if CH3 is present at the surface of implants, this chemical group will trigger a significant enhancement of activated phagocytes involved in the inflammatory reaction, and this phenomenon may extend the local phlogistic event.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7