Effects of dimethoate and beta-naphthoflavone on selected biomarkers of Poecilia reticulata

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Manuela F Frasco (Autor) Outra; Guilhermino, L. (Autor) ICBAS

Revista

Título: Fish Physiology and Biochemistry

Vol. 26

Páginas: 149-156

ISSN: 0920-1742

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 116 Citações

Scopus - 122 Citações

Outras Informações

ID Authenticus: P-000-R67

DOI: 10.1023/a:1025457831923

Abstract (EN): The main objective of the present study was to investigate if a battery of enzymatic biomarkers was suitable for use as effect criteria in acute toxicity tests with Poecilia reticulata. To attain this objective, the in vivo effects of dimethoate and beta-naphthoflavone on acetylcholinesterase (AChE), cytochrome P4501A-dependent monooxygenase activity of 7-ethoxyresorufin O-deethylase (EROD), glutathione S-transferases (GST), lactate dehydrogenase (LDH) and Na+-K+-ATPase activities of P. reticulata were studied. After 96 h of exposure to sublethal concentrations (0.063 mg l(-1) to 1 mg l(-1)) of the pesticide, an inhibition of the enzymes AChE and GST, as well as an induction of LDH was observed. The compound beta-naphthoflavone significantly induced both EROD and GST. The remaining enzymes analysed were not significantly altered by the exposure to beta-naphthoflavone (0.82 mg l(-1) to 1.7 mg l(-1)). These results suggest that in vivo toxicity tests based on the biomarkers used in this study are sensitive and present advantages to conventional acute tests based on mortality, since they were able to detect sublethal effects in a short-period of time (96 h) indicating target and/or detoxification mechanisms.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8