Sialyl Lewis(x)-dependent binding of human monocyte-derived dendritic cells to selectins

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Silva, Z (Autor) Outra; Tong, ZQ (Autor) Outra; Guadalupe Cabral, MG (Autor) Outra; Martins, C (Autor) Outra; Castro, R (Autor) Outra; Celso Reis (Autor) ICBAS; Trindade, H (Autor) Outra; Konstantopoulos, K (Autor) Outra; Videira, PA (Autor) Outra

Revista

Título: Biochemical and Biophysical Research Communications

Vol. 409

Páginas: 459-464

ISSN: 0006-291X

Editora: Elsevier

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-002-QGV

DOI: 10.1016/j.bbrc.2011.05.026

Abstract (EN): The limited efficacy of monocyte-derived dendritic cell (mo-DC)-based vaccines is primarily attributed to the reduced mo-DC migratory capacity. One undefined aspect is the initial binding of mo-DCs to endothelial cells and vascular selectins. In this study, we investigated the role and modulation of the selectin binding determinant sialyl Lewis(x) (sLe(x)) in selectin-dependent mo-DC binding. Our data reveal that sLe(x) is required for maximal binding of mo-DCs to tumor necrosis factor (TNF)-alpha-activated endothelial cells under static conditions, as evidenced by the use of sialidase. Sialidase treatment also abrogated mo-DC cell tethering to immobilized, purified P-, L-, or E-selectin under flow. The requirement of sLe(x)-dependent binding of mo-DC to selectins was further substantiated by using sLe(x) free sugar and anti-sLe(x) antibody, which significantly suppressed mo-DC-selectin binding. P-selectin glycoprotein ligand-1 is required for mo-DC binding to both P- and L-selectin, but it is dispensable for E-selectin recognition. Interestingly, the extent of mo-DC tethering was maximal on P-selectin, followed by E- and L- selectin. Accordingly, L-selectin mediated faster mo-DC rolling than E- or P-selectin. Interferon (IFN)-gamma induces a significant increase in mo-DC surface sLe(x\) expression, which is probably due to the enhanced synthesis of C2GnT-I. These findings may contribute to improving mo-DC-based vaccination protocols.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6