Long-term exercise training as a modulator of mammary cancer vascularization

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

A. I. Faustino Rocha (Autor) Outra; A. Silva (Autor) Outra; J. Gabriel (Autor) FEUP; R. M. Gil da Costa (Autor) FEUP; M. Moutinho (Autor) Outra; P. A. Oliveira (Autor) Outra; A. Gama (Autor) Outra; R. Ferreira (Autor) Outra; M. Ginja (Autor) Outra

Revista

Título: Biomedicine & Pharmacotherapy

Vol. 81

Páginas: 273-280

ISSN: 0753-3322

Editora: Elsevier

Indexação

ISI Web of Knowledge - 19 Citações

Scopus - 22 Citações

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00K-DJ7

DOI: 10.1016/j.biopha.2016.04.030

Abstract (EN): Background: Breast cancer remains a leading cause of death by cancer worldwide. It is commonly accepted that angiogenesis and the expression of angiogenic factors such as vascular endothelial growth factor-A (VEGF-A) is associated with the increased risk of metastasis and poor patient outcome. Objective: This work aimed to evaluate the effects of long-term exercise training on the growth and vascularization of mammary tumors in a rat model. Materials and methods: Fifty female Sprague-Dawley rats were divided into four groups: two N-methyl-N-nitrosourea (MNU)-exposed groups (exercised and sedentary) and two control groups (exercised and sedentary). MNU was administered once, intraperitoneally at 7 weeks-old. Animals were then exercised on a treadmill for 35 weeks. Mammary tumors were evaluated using thermography, ultrasonography [Power Doppler (PDI), B Flow and contrast-enhanced ultrasound (CEUS)], and immunohistochemistry (VEGF-A). Results: Both, MNU sedentary and exercised groups showed 100% of tumor incidence, but exercised animals showed less tumors with an increased latency period. Exercise training also enhanced VEGF-A immunoexpression and vascularization (microvessel density, MVD) (p < 0.05), and reduced histological aggressiveness. Ultrasound and thermal imaging analysis confirmed the enhanced vascularization of tumors on exercised animals. Conclusion: Long-term exercise training increased VEGF-A expression, leading to enhanced tumor vascularization and reduced tumor burden, multiplicity and histological aggressiveness. © 2016 Elsevier Masson SAS.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8