Development of an automatic identification algorithm for antibiogram analysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Luan F. R. Costa (Autor) Outra; Eduardo S. da Silva (Autor) FEUP; Victor T. Noronha (Autor) Outra; Ivone Vaz Moreira (Autor) FEUP; Olga C. Nunes (Autor) FEUP; Marcelino M. de Andrade (Autor) Outra

Revista

Título: Computers in Biology and Medicine

Vol. 67

Páginas: 104-115

ISSN: 0010-4825

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 2 Citações

INSPEC

Projetos Associados

LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

Outras Informações

ID Authenticus: P-00G-SDJ

DOI: 10.1016/j.compbiomed.2015.09.020

Abstract (EN): Routinely, diagnostic and microbiology laboratories perform antibiogram analysis which can present some difficulties leading to misreadings and intra and inter-reader deviations. An Automatic Identification Algorithm (AIA) has been proposed as a solution to overcome some issues associated with the disc diffusion method, which is the main goal of this work. ALA allows automatic scanning of inhibition zones obtained by antibiograms. More than 60 environmental isolates were tested using susceptibility tests which were performed for 12 different antibiotics for a total of 756 readings. Plate images were acquired and classified as standard or oddity. The inhibition zones were measured using the AIA and results were compared with reference method (human reading), using weighted kappa index and statistical analysis to evaluate, respectively, inter-reader agreement and correlation between AIA-based and human-based reading. Agreements were observed in 88% cases and 89% of the tests showed no difference or a <4 mm difference between AIA and human analysis, exhibiting a correlation index of 0.85 for all images, 0.90 for standards and 0.80 for oddities with no significant difference between automatic and manual method. AIA resolved some reading problems such as overlapping inhibition zones, imperfect microorganism seeding, non-homogeneity of the circumference, partial action of the antimicrobial, and formation of a second halo of inhibition. Furthermore, ALA proved to overcome some of the limitations observed in other automatic methods. Therefore, AIA may be a practical tool for automated reading of antibiograms in diagnostic and microbiology laboratories.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12