ST6GalNAc-I controls expression of sialyl-Tn antigen in gastrointestinal tissues

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Marcos, NT (Autor) Outra; Bennett, EP (Autor) Outra; Gomes, J (Autor) Outra; Magalhaes, A (Autor) Outra; Gomes, C (Autor) Outra; David L (Autor) FMUP; Dar, I (Autor) Outra; Jeanneau, C (Autor) Outra; DeFrees, S (Autor) Outra; Krustrup, D (Autor) Outra; Vogel, LK (Autor) Outra; Kure, EH (Autor) Outra; Burchell, J (Autor) Outra; Taylor Papadimitriou, J (Autor) Outra; Clausen, H (Autor) Outra; Mandel, U (Autor) Outra; Celso Reis (Autor) ICBAS

Revista

Título: Frontiers in Bioscience - Elite

Vol. 3 E

Páginas: 1443-1455

ISSN: 1945-0494

Editora: Frontiers in Bioscience

Indexação

Scopus - 90 Citações

Outras Informações

ID Authenticus: P-008-0K6

Abstract (EN): Sialyl-Tn is a simple mucin-type carbohydrate antigen aberrantly expressed in gastrointestinal adenocarcinomas and in the precursor lesion intestinal metaplasia. Sialyl-Tn tumour expression is an independent indicator of poor prognosis. We have previously shown in vitro that ST6GalNAc-I and ST6GalNAc-II sialyltransferases can synthesize sialyl-Tn. The aim of the present study was to establish whether ST6GalNAc-I is the major enzyme responsible for the expression of sialyl-Tn. We used a model of CHO-ldlD cells producing only MUC1-Tn glycoform and showed that ST6GalNAc-I is the key-enzyme leading to sialyl-Tn biosynthesis. We developed novel monoclonal antibodies specific for ST6GalNAc-I and evaluated its expression in gastrointestinal tissues. ST6GalNAc-I was detected in normal colon mucosa co-localized with O-acetylated sialyl-Tn. Expression was largely unaltered in colorectal adenocarcinomas. In contrast, we found that ST6GalNAc-I is weakly expressed in normal gastric mucosa, but overexpressed in intestinal metaplasia, co-localized with sialyl-Tn. In gastric carcinomas ST6GalNAc-I was also associated with sialyl-Tn, but with heterogeneous staining and partial co-localization. Our results showed ST6GalNAc-I as the major enzyme controlling the expression of cancer-associated sialyl-Tn antigen in gastrointestinal tissues.

Idioma: Inglês

Tipo (Avaliação Docente): Científica