Microarray based IgE detection in poly-sensitized allergic patients with suspected food allergy - an approach in four clinical cases

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Vieira, T (Autor) Outra; Lopes, C (Autor) FMUP; Pereira, AM (Autor) Outra; Araujo, L (Autor) FMUP; Moreira, André (Autor) FMUP; Delgado, L (Autor) FMUP

Revista

Título: Allergologia et Immunopathologia

Vol. 40

Páginas: 172-180

ISSN: 0301-0546

Editora: Elsevier

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 19 Citações

Outras Informações

ID Authenticus: P-002-AEW

DOI: 10.1016/j.aller.2011.03.013

Abstract (EN): Background: Component-resolved diagnosis and microarray technology have been recently introduced into clinical allergy practice, and may be particularly useful in poly-sensitized allergic patients. Methods: We compare the clinical usefulness of a microarray-based IgE detection assay (ISAC (R)) with skin tests and specific IgE with standard allergens (sIgE) or their monocomponents in four case reports of patients poly-sensitized to aeroallergens and food. Results: Case 1: a woman with rhinitis, oral allergy syndrome to several fruits and anaphylaxis to cherry. Diagnostic tests supported non-specific lipid transfer proteins (nsLTPs) primary sensitization. Case 2: a woman with exercise-induced asthma, rhino-conjunctivitis and oral allergy syndrome to fresh fruits of different families. A diagnosis of primary grass and weed pollen allergy with profilin and pathogenesis-related protein family 10 (PR-10) cross-reactive food allergy was proposed. Case 3: a man with atopic eczema, asthma, rhinitis, and multiple anaphylactic episodes with cashew nuts and oral allergy syndrome to fruits. The diagnostic workup supported a primary birch pollen allergy with PR-10 and nsLTPs cross-reactive food allergy. Case 4: a woman with rhino-conjunctivitis, per-operative anaphylaxis due to latex and recent pharyngeal angio-oedema episodes. The diagnosis was a primary grass and weed pollen allergy with equivocal profilin sensitization and no obvious cross-reactivity mediated by nsLTPs sensitization. Conclusions: The possibility to carry out multiple sIgE measurements with single protein allergens, in particular with the microarray technique, is a useful, simple and non-invasive diagnostic tool in complex poly-sensitized allergic patients.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9