Helicobacter pylori cag pathogenicity island-positive strains induce syndecan-4 expression in gastric epithelial cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Magalhães A (Autor) Outra; Marcos NT (Autor) Outra; Carvalho AS (Autor) Outra; David L (Autor) FMUP; Figueiredo C (Autor) FMUP; Bastos J (Autor) Outra; David G (Autor) Outra; Reis CA (Autor) FEUP

Revista

Título: FEMS Immunology and Medical Microbiology

Vol. 56 Nº 3

Páginas: 223-232

ISSN: 0928-8244

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 12 Citações

Pubmed / Medline

Outras Informações

ID Authenticus: P-003-GZY

DOI: 10.1111/j.1574-695x.2009.00569.x

Abstract (EN): Helicobacter pylori is recognized as the main cause of gastritis and is associated with gastric carcinogenesis. Syndecan-4 represents the major source of heparan sulfate (HS) in the gastric cells. HS proteoglycans expressed on the cell surface constitute targets for H. pylori at the early stage of infection. The aim of this study was to determine whether H. pylori induction of syndecan-4 expression is affected by the virulence characteristics of the infecting strain, namely the cytotoxic-associated gene (cag) pathogenicity island (PAI). We observed that individuals infected with highly pathogenic H. pylori strains express syndecan-4 in the foveolar epithelium of the gastric mucosa. The association between the cagPAI status of the infecting strain and syndecan-4 expression was further demonstrated by infection of gastric epithelial cell lines with a panel of cagPAI(+) and cagPAI(-) H. pylori strains, showing that expression of syndecan-4 was significantly increased in response to infection with the highly pathogenic strains. Moreover, infection of gastric cells with cagA and cagE mutant strains further confirmed that syndecan-4 induction is dependent on an intact cagPAI. The present study shows that highly pathogenic H. pylori strains induce syndecan-4 expression, both in human gastric mucosa and in gastric cell lines, in a cagPAI-dependent manner.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10