Aldobiouronic acid domains in Helicobacter pylori

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Ferreira, JA (Autor) Outra; Domingues, MRM (Autor) Outra; Reis, A (Autor) Outra; Figueiredo C (Autor) FMUP; Monteiro, MA (Autor) Outra; Coimbra, MA (Autor) Outra

Revista

Título: Carbohydrate Research

Vol. 346

Páginas: 638-643

ISSN: 0008-6215

Editora: Elsevier

Indexação

ISI Web of Knowledge - 8 Citações

Scopus - 8 Citações

Outras Informações

ID Authenticus: P-002-SS1

DOI: 10.1016/j.carres.2011.01.009

Abstract (EN): Helicobacter pylori cell-surface glycans exert strong influences in host-microbe interplays and define the strain's immunological signature. Envisaging the development of a carbohydrate-based vaccine against the gastroduodenal pathogen H. pylon, several clinical isolates are being screened for their cell-surface glycan profile. The present work concerns H. pylori clinical specimen PTAV79 that abundantly expressed amylose-like glycans. These polysaccharides were isolated in glycan-rich fractions resultant from phenol-water extractions and purified by Bio-Gel P2. Structural studies showed that the glycans are linked to glycerol and present aldobiouronic acid domains composed of [-> 3)-alpha-D-GlcA-(1 -> 4)-alpha-D-Glc-(1 ->] repeating units. The amylose domains were constituted by an average of 19 Glc residues and the acidic moieties had an average number of 10 aldobiouronic acid repeating units. These polysaccharides were isolated in fractions that, although hydrophilic, were rich in stearic acid, strongly suggesting that they are present as glycerolipids anchored to cell-surface.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6