Optimization of processing conditions for the quantification of enterococci biofilms using microtitre-plates

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Extremina, CI (Autor) Outra; Costa, L (Autor) Outra; Aguiar, AI (Autor) Outra; Peixe, L (Autor) FFUP; Fonseca, AP (Autor) Outra

Revista

Título: Journal of Microbiological Methods

Vol. 84

Páginas: 167-173

ISSN: 0167-7012

Editora: Elsevier

Indexação

ISI Web of Knowledge - 30 Citações

Scopus - 41 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-VA7

DOI: 10.1016/j.mimet.2010.11.007

Abstract (EN): We performed a comparison of four selected protocols from the literature using microtitre-plate and colorimetric biomass assay for evaluation of enterococci biofilm formation ability and optimized a protocol after the identification and stringent performance of biofilm formation steps. The optimized protocol uses a dynamic model that provided a greater discrimination of enterococci biofilm formation ability, and could better simulate in vivo real conditions. Moreover different biofilm quantification approaches, such as the colorimetric biomass (crystal violet), the resazurin and CFU's assays could be used with the optimized protocol, with adequate reproducibility. This study also recognizes that parameters such as the biofilm formation index (BFi), the cut-off values and the Z' factor provide greater accuracy, possibility of inter and intra laboratory comparison and quality evaluation of the biofilm screening assays, respectively.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7