Molecular characterization of bla(IMP-5), a new integron-borne metallo-beta-lactamase gene from an Acinetobacter baumannii nosocomial isolate in Portugal

• Tipo: Artigo em Revista Científica Internacional
• Data: 2002

Autores

Da Silva, GJ (Autor) Outra; Correia, M (Autor) Outra; Vital, C (Autor) Outra; Ribeiro, G (Autor) Outra; Sousa, JC (Autor) Outra; Leitao, R (Autor) Outra; Peixe, L (Autor) FFUP; Duarte, A (Autor) Outra

Revista

Título: FEMS Microbiology Letters

Vol. 215

Páginas: 33-39

ISSN: 0378-1097

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 77 Citações

Scopus - 92 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-MYW

DOI: 10.1016/s0378-1097(02)00896-0

Abstract (EN): Acinetobacter baumannii 65FFC, an imipenem-resistant clinical strain, isolated from the urine of a patient at the Coimbra University Hospital, Portugal, in 1998, produced a metallo-beta-lactamase with a calculated pI 9.3. The isolate was highly resistant to penicillins, broad-spectrum cephalosporins, including ceftazidime, ceftriaxone, cefepime, cefpirome, and to aztreonam, but it remained susceptible to ampicillin/sulbactam, aminoglycosides and quinolones. Nucleotide sequence revealed a new allelic variant of other bla(IMP) genes, named bla(IMP-5). IMP-5 beta-lactamase showed a greater homology with IMP-1, IMP-3 and IMP-4 (identified in Southeast Asia), than with IMP-2, found in Italy (93%, 92%, 91% and 87% of amino acid identity, respectively). bla(IMP-5) was the only gene cassette inserted into a class I integron, named In76. This is the first IMP-enzyme reported in Portugal and the second in Europe, indicating a wider dissemination in the environment of bla(IMP) alleles.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7