Surface functionalization of polymeric nanospheres modulates macrophage activation: relevance in Leishmaniasis therapy

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Daniela Barros (Autor) Outra; Sofia A C Costa Lima (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP

Revista

Título: Nanomedicine

Vol. 10

Páginas: 387-403

ISSN: 1743-5889

Editora: Future Medicine Ltd.

Indexação

ISI Web of Knowledge - 14 Citações

Scopus - 29 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Nanotecnologia

Outras Informações

ID Authenticus: P-00A-785

DOI: 10.2217/nnm.14.116

Abstract (EN): Aim: To characterize the production and application of carbohydrate functionalized poly(d,l-lactide-co-glycolide) (PLGA) nanospheres as immune-modulatory mediators in the treatment of visceral leishmaniasis (VL). Materials & methods: PLGA nanospheres were prepared by nanoprecipitation and surface functionalized with mannose, mannan or mannosamine moieties using a carbodiimide reaction. Flow cytometry and fluorescence microscopy revealed the interaction of these nanospheres with macrophages. Results: The nanocarriers were taken up by murine primary macrophages using clathrin-mediated endocytosis. Co-culture of macrophages with carbohydrate-functionalized nanospheres led to their activation and production of pro-inflammatory cytokines. One dose of amphotericin B-loaded on mannan-functionalized nanospheres resulted in an efficacy VL therapy. Conclusion: This approach provides a promising therapy for VL and a new therapeutic nanoplatform for obligate intracellular pathogens.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17