Effect of Cell Density on Mesenchymal Stem Cells Aggregation in RGD-Alginate 3D Matrices under Osteoinductive Conditions

• Tipo: Artigo em Revista Científica Internacional
• Data: 2014

Autores

Raquel Maia, FR (Autor) Outra; Ana H Lourenco (Autor) Outra; Pedro L Granja (Autor) FEUP; Raquel M Goncalves (Autor) Outra; Cristina C Barrias (Autor) Outra

Revista

Título: Macromolecular Bioscience

Vol. 14

Páginas: 759-771

ISSN: 1616-5187

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 49 Citações

Scopus - 53 Citações

Outras Informações

ID Authenticus: P-009-JKG

DOI: 10.1002/mabi.201300567

Abstract (EN): Cellular activities in 3D are differentially affected by several matrix-intrinsic and extrinsic factors. This study highlights the relevance of optimizing initial cell densities when establishing 3D cultures for specific applications. Independently of the entrapping density, MSCs cultured within RGD-alginate hydrogels showed steady-state levels of metabolic activity and were in a nearly non-proliferative state, but recovered "normal'' activity levels when retrieved from 3D matrices and re-cultured as monolayers. Importantly, high-densities promoted the establishment of cell-cell contacts with formation of multicellular clusters stabilized by endogenous ECM, and also stimulated MSCs osteogenic differentiation. These MSC-ECM microtissues may be used as building blocks for tissue engineering.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13