Human periprostatic white adipose tissue is rich in stromal progenitor cells and a potential source of prostate tumor stroma

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Ricardo Ribeiro (Autor) Outra; Catia Monteiro (Autor) Outra; Ricardo Silvestre (Autor) Outra; Angela Castela (Autor) Outra; Helena Coutinho (Autor) Outra; Avelino Fraga (Autor) Outra; Paulo Principe (Autor) Outra; Carlos Lobato (Autor) Outra; Carla Costa (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP; Jose Manuel Lopes (Autor) FMUP; Carlos Lopes (Autor) Outra; Rui Medeiros (Autor) ICBAS

Revista

Título: Experimental Biology and Medicine

Vol. 237 Nº 8

Páginas: 1155-1162

ISSN: 1535-3702

Editora: SAGE

Indexação

ISI Web of Knowledge - 28 Citações

Scopus - 28 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Outras ciências médicas

Outras Informações

ID Authenticus: P-002-4N0

DOI: 10.1258/ebm.2012.012131

Abstract (EN): A body of growing evidence now implicates white adipose tissue as a relevant source of stromal progenitor cells recruited to the tumor microenvironment to form supportive tumor stroma. While the role of periprostatic (PP) adipose tissue in prostate cancer progression has been barely appreciated, we sought to determine the progenitor cell population in PP adipose tissue and the association with prostate cancer. We isolated and characterized CD31(-)CD34(+)CD45(-)CD0146(-) progenitor cells (adipose-derived stem cells [ASC]) in paired samples of PP and preperitoneal visceral adipose tissue from prostate tissue and peripheral blood mononuclear cells of prostate cancer and nodular prostatic hyperplasia patients. ASC were quantified by flow cytometry and confirmed through target gene expression. Here we show a significantly higher amount of ASC in PP than in visceral adipose tissue, independent of body mass index and prostatic disease. In the prostate, ASC are increased in cancer compared with prostatic nodular hyperplasia patients. Concordantly, adipsin gene (CFD) expression, which is known to be up-regulated in adipose stem cells, was overexpressed in PP adipose tissue, in the prostate of cancer patients and in prostate CD31(-)CD34(+)CD45(-)CD146(-) sorted cells. ASC were found at higher levels in the blood of prostate cancer patients simultaneously overweight/obese. Present findings indicate that PP adipose tissue is a reservoir of progenitor cells with the potential to migrate towards prostate tumors, although its clinical significance merits further evaluation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8