Dynamics of in vitro acquisition of resistance by Candida parapsilosis to different azoles

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

silva, atpe (Autor) Outra; costa-de-oliveira, s (Autor) FMUP; silva-dias, a (Autor) Outra; pina-vaz, c (Autor) FMUP; rodrigues, ag (Autor) FMUP

Revista

Título: FEMS Yeast Research

Vol. 9

Páginas: 626-633

ISSN: 1567-1356

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 30 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-003-JGK

DOI: 10.1111/j.1567-1364.2009.00508.x

Abstract (EN): Candida parapsilosis is a common isolate from clinical fungal infectious episodes. Resistance of C. parapsilosis to azoles has been increasingly reported. To analyse the development of resistance in C. parapsilosis, four azole-susceptible clinical strains and one American Type Culture Collection type strain were cultured in the presence of fluconazole, voriconazole and posaconazole at different concentrations. The isolates developed variable degrees of azole resistance according to the antifungal used. Fluconazole was the fastest inducer while posaconazole was the slowest. Fluconazole and voriconazole induced resistance to themselves and each other, but not to posaconazole. Posaconazole induced resistance to all azoles. Developed resistance was stable; it could be confirmed after 30 days of subculture in drug-free medium. Azole-resistant isolates revealed a homogeneous population structure; the role of azole transporter efflux pumps was minor after evaluation by microdilution and cytometric assays with efflux pump blockers (verapamil, ibuprofen and carbonyl cyanide 3-chloro-phenylhydrazone). We conclude that the rapid development of azole resistance occurs by a mechanism that might involve mutation of genes responsible for ergosterol biosynthesis pathway, stressed by exposure to antifungals.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8