Evaluation of the sensitivity of genotoxicity and cytotoxicity endpoints in earthworms exposed in situ to uranium mining wastes

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Joana Lourenço (Autor) Outra; Ruth Pereira (Autor) FCUP; Ana Silva (Autor) Outra; Fernando Carvalho (Autor) Outra; João Oliveira (Autor) Outra; Margarida Malta (Autor) Outra; Artur Paiva (Autor) Outra; Fernando Gonçalves (Autor) Outra; Sónia Mendo (Autor) Outra

Revista

Título: Ecotoxicology and Environmental Safety

Vol. 75

Páginas: 46-54

ISSN: 0147-6513

Editora: Elsevier

Indexação

ISI Web of Knowledge - 34 Citações

Scopus - 36 Citações

Outras Informações

ID Authenticus: P-002-HJ8

DOI: 10.1016/j.ecoenv.2011.08.024

Abstract (EN): Earthworms were exposed for 56 days to a contaminated soil from an abandoned uranium mine and to the natural reference soil LUFA 2.2. The exposure occurred in situ: the containers with contaminated soil were placed near the mine pit; the containers with reference soil were placed in a reference site. For the assessment of metals bioaccumulation, DNA damages, cell-to-cell variation in DNA content, Median Fluorescence Intensity (MFI), coelomocytes frequency and proliferation, organisms were sampled after 0, 1, 2, 7, 14 and 56 days of exposure. For the assessment of radionuclides bioaccumulation, animals were sampled after 0, 14 and 56 days of exposure. As for growth, organisms were sampled after 0, 14, 28 and 56 days of exposure. The reproduction assay was performed according to the OECD (2004) guideline. DNA damages were assessed by comet assay and flow cytometry was used to determine cell-to-cell variation in DNA content, Median Fluorescence Intensity (MFI), coelomocytes frequency and proliferation. Results have shown a myriad of effects in the organisms exposed to the contaminated soil, namely: the inhibition of reproduction, growth reduction, DNA damages, cytotoxicity, changes in eleocytes fluorescence intensity, coelomocytes proliferation and bioaccumulation of metals and radionuclides. Our results showed that the evaluation of genotoxicity and cytotoxicity endpoints, along with other parameters at an individual level in standard reproduction assays conducted in situ, are important to improve the risk assessment process of areas contaminated with uranium and other radioactive mining wastes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9