Analysis of salivary peptides using HPLC-electrospray mass spectrometry

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

vitorino, r (Autor) Outra; lobo, mjc (Autor) Outra; duarte, jar (Autor) FADEUP; ferrer-correia, aj (Autor) Outra; domingues, pm (Autor) Outra; amado, fml (Autor) Outra

Revista

Título: Biomedical Chromatography

Vol. 18

Páginas: 570-575

ISSN: 0269-3879

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 35 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-865

DOI: 10.1002/bmc.358

Abstract (EN): Salivary peptides are involved in a wide range of functions constituting the first line of defence of oral cavity and precursors of dental pellicle formation. The presence of mucins in saliva makes difficult the analysis of the proteic content. This is due mainly to aggregation phenomenon between mucins and other high molecular weight glycoproteins and salivary proteins. Considering the importance of salivary peptides in biological functions, we have evaluated the influence of four different extraction methodologies on the separation and identification of these proteins by HPLC-MS. Based on their molecular weight, we identified a total of 22 peptides when extraction was performed using a solution of guanidine (6 M), compared with 14 peptides identified when saliva is acidified with TFA, which is an often used procedure. Our results also show the presence of mucin bind peptides, which include statherin, PRP1, PRP3, Histatin 1 and Histatin 5. Copyright (C) 2004 John Wiley Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6