Albumin adsorption on alkanethiols self-assembled monolayers on gold electrodes studied by chronopotentiometry

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

M. C. L. Martins (Autor) Outra; José Carlos Fonseca (Autor) FEUP; Mário Adolfo Barbosa (Autor) FEUP; B. D. Ratner (Autor) Outra

Revista

Título: Biomaterials

Vol. 24 Nº 21

Páginas: 3697-3706

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 45 Citações

Scopus - 48 Citações

Outras Informações

ID Authenticus: P-000-F7R

DOI: 10.1016/s0142-9612(03)00244-8

Abstract (EN): Chronopotentiometry was used to study the adsorption of human serum albumin (HSA) to self-assembled monolayers with the following terminal functional groups: CH3, COOH and OH. Surfaces were characterized by X-ray photoelectron spectroscopy, water contact angle measurements and cyclic voltammetry. HSA coverage of the different SAMs was investigated by chronopotentiometry and the total amount of adsorbed protein was determined using radiolabelled albumin. Both techniques have demonstrated that HSA adsorption to the different SAM-modified electrodes increases in the following order: OH < COOH < CH3-terminated SAMs. A good correlation between coverage and total amount of HSA adsorbed was observed for long adsorption times (900 s).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10