Fut2-null mice display an altered glycosylation profile and impaired BabA-mediated Helicobacter pylori adhesion to gastric mucosa

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Magalhaes, A (Autor) Outra; Gomes, J (Autor) Outra; Ismail, MN (Autor) Outra; Haslam, SM (Autor) Outra; Mendes, N (Autor) Outra; Osorio, H (Autor) Outra; David L (Autor) FMUP; Le Pendu, J (Autor) Outra; Haas, R (Autor) Outra; Dell, A (Autor) Outra; Boren, T (Autor) Outra; Celso Reis (Autor) ICBAS

Revista

Título: Glycobiology

Vol. 19 Nº 12

Páginas: 1525-1536

ISSN: 0959-6658

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 78 Citações

Scopus - 84 Citações

Outras Informações

ID Authenticus: P-003-DXM

DOI: 10.1093/glycob/cwp131

Abstract (EN): Glycoconjugates expressed on gastric mucosa play a crucial role in host-pathogen interactions. The FUT2 enzyme catalyzes the addition of terminal alpha(1,2)fucose residues, producing the H type 1 structure expressed on the surface of epithelial cells and in mucosal secretions of secretor individuals. Inactivating mutations in the human FUT2 gene are associated with reduced susceptibility to Helicobacter pylori infection. H. pylori infects over half the world's population and causes diverse gastric lesions, from gastritis to gastric cancer. H. pylori adhesion constitutes a crucial step in the establishment of a successful infection. The BabA adhesin binds the Le(b) and H type 1 structures expressed on gastric mucins, while SabA binds to sialylated carbohydrates mediating the adherence to inflamed gastric mucosa. In this study, we have used an animal model of nonsecretors, Fut2-null mice, to characterize the glycosylation profile and evaluate the effect of the observed glycan expression modifications in the process of H. pylori adhesion. We have demonstrated expression of terminal difucosylated glycan structures in C57Bl/6 mice gastric mucosa and that Fut2-null mice showed marked alteration in gastric mucosa glycosylation, characterized by diminished expression of alpha(1,2)fucosylated structures as indicated by lectin and antibody staining and further confirmed by mass spectrometry analysis. This altered glycosylation profile was further confirmed by the absence of Fuc alpha(1,2)-dependent binding of calicivirus virus-like particles. Finally, using a panel of H. pylori strains, with different adhesin expression profiles, we have demonstated an impairment of BabA-dependent adhesion of H. pylori to Fut2-null mice gastric mucosa, whereas SabA-mediated binding was not affected.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12