Targetability of hyaluronic acid nanogel to cancer cells: In vitro and in vivo studies

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Pedrosa, SS (Autor) Outra; Pereira, P (Autor) Outra; Alexandra Correia (Autor) FCUP; Gama, FM (Autor) Outra

Revista

Título: European Journal of Pharmaceutical Sciences

Vol. 104

Páginas: 102-113

ISSN: 0928-0987

Editora: Elsevier

Indexação

ISI Web of Knowledge - 31 Citações

Scopus - 34 Citações

Outras Informações

ID Authenticus: P-00M-NEK

DOI: 10.1016/j.ejps.2017.03.045

Abstract (EN): We have, in previous work developed, characterized and evaluated the biocompatibility of an engineered hyaluronic acid nanogel. Here we assess the targetability of a hyaluronic acid nanogel towards CD44 overexpressing cells, in vitro and in vivo. Results obtained by flow cytometry and confocal fluorescence microscopy shows that nanogel is greatly internalized by non-small cancer lung cells (A549 cells), that overexpress CD44 receptors. The biodistribution and tumor targetability of the nanogel labelled with a near infrared (NIR) probe were performed, in mice, through a non-invasive imaging system. Results revealed nanogel high targetability towards an induced subcutaneous A549 tumor. Nanogels pharmacoldnetics was evaluated also in healthy animals, and Alexa Fluor 680 labelled nanogel exhibited higher accumulation in liver, kidneys and skin. Also, a comparative biodistribution study was performed, using two NIR imaging probes, Cy5.5 and Alexa Fluor 680.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12