Experimental characterization and modelling of analytical monolithic column

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Zabka, M (Autor) Outra; Minceva, M (Autor) Outra; Alírio Rodrigues (Autor) FEUP

Revista

Título: Journal of Biochemical and Biophysical Methods

Vol. 70 Nº 1

Páginas: 95-105

ISSN: 0165-022X

Editora: Elsevier B.V.

Indexação

ISI Proceedings

ISI Web of Knowledge - 15 Citações

Scopus - 13 Citações

Classificação Científica

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia química

Outras Informações

ID Authenticus: P-004-BRX

DOI: 10.1016/j.jbbm.2006.10.002

Resumo (PT): PHASE LIQUID-CHROMATOGRAPHY; SIZE-EXCLUSION CHROMATOGRAPHY; POROUS SILICA COLUMNS; STATIONARY PHASES; BAND PROFILES; ENANTIOMER SEPARATIONS; PROTEIN SEPARATION; BASIC COMPOUNDS; PORE STRUCTURE; PRESSURE-DROP

Abstract (EN): Hydrodynamics, equilibrium and kinetics of adsorption in a silica-based monolithic column Chromolith Performance RP-18e (Merck KgaA, Germany) have been studied. The column permeability was calculated according to the Darcy law for laminar flow. The efficiency of the monolithic column was characterized through the height equivalent to a theoretical plate (HETP) for myoglobin, phenol and progesterone. The 2-D single channel mathematical model has been applied to describe the adsorption dynamics. Parabolic velocity profile, axial and radial diffusion in the monolith channel, linear driving force model for the mass transfer in the monolith channel skeleton wall and linear adsorption equilibrium were assumed. The mathematical model gives good prediction of the experimental elution peaks.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11