Serine-based surfactants as effective antimicrobial agents against multiresistant bacteria

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Sandra G. Silva (Autor) FCUP; Pinheiro, M (Autor) Outra; Pereira, R (Autor) Outra; Dias, AR (Autor) Outra; Ferraz, R (Autor) Outra; Prudencio, C (Autor) Outra; Eaton, PJ (Autor) Outra; Salette Reis (Autor) FFUP; M. Luisa C. do Vale (Autor) FCUP

Revista

Título: Biochimica et Biophysica Acta: Biomembranes

Vol. 1864

ISSN: 0005-2736

Editora: Elsevier

Indexação

ISI Web of Knowledge - 1 Citação

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00W-MGY

DOI: 10.1016/j.bbamem.2022.183969

Abstract (EN): The antimicrobial activity of two serine derived gemini cationic surfactants, amide (12Ser)2CON12 and ester (12Ser)2COO12, was tested using sensitive, E. coli ATCC 25922 and S. aureus ATCC 6538, and resistant, E. coli CTX M2, E. coli TEM CTX M9 and S. aureus ATCC 6538 and S. aureus MRSA ATCC 43300 Gram-positive and Gram-negative bacteria strains. Very low MIC values (5 mu M) were found for the two resistant strains E.coli TEM CTX M9 and S. aureus MRSA ATCC 43300, in the case of the amide derivative, and for S. aureus MRSA ATCC 43300, in the case of the ester derivative. The interaction of the serine amphiphiles with lipid-model membranes (DPPG and DPPC) was investigated using Langmuir monolayers. A more pronounced effect on the DPPG than on the DPPC monolayer was observed. The effect induced by the surfactants on bacteria membrane was explored by Atomic Force Microscopy. A clear disruption of the bacteria membrane was observed for E. coli TEM CTX M9 upon treatment with (12ser)2CON12, whereas for the S. aureus MRSA few observable changes in cell morphology were found after treatment with either of the two surfactants. The cytotoxicity of the two compounds was assessed by hemolysis assay on human red blood cells (RBC). The compounds were shown to be non-cytotoxic up to 10 mu M. Overall, the results reveal a promising potential, in particular of the amide derivative, as antimicrobial agent for two strains of antibiotic resistant bacteria.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10