The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Maria João Romeu (Autor) FEUP; Lima, M (Autor) Outra; Luciana Gomes (Autor) FEUP; de Jong, ED (Autor) Outra; Morais, J (Autor) Outra; Vitor Vasconcelos (Autor) FCUP; Pereira, MFR (Autor) Outra; Soares, OSGP (Autor) Outra; Sjollema, J (Autor) Outra; Filipe Mergulhão (Autor) FEUP

Revista

Título: Polymers

Vol. 11 Nº 5 Article number 923

Página Final: 4410

Editora: MDPI

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 6 Citações

Projetos Associados

ALiCE - ALiCE - Laboratório Associado em Engenharia Química

LEPABE - Financiamento Plurianual 2020-2023 da Unidade de I&D LEPABE - Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia

NanoCAT - NanoCAT - Utilização de nanotubos de carbono modificados para combater infecções em catéteres urinários e stents

SurfSAFE - Surface modification to increase microbial SAFEty in the food industry

Healthy Waters - Healthy Waters - Identification, Elimination, Social Awareness and Education of Water Chemical and Biological Micropollutants with Health and Environmental Implications

Outras Informações

ID Authenticus: P-00X-BFD

DOI: 10.3390/polym14204410

Abstract (EN): The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different scenarios. In this study, the effect of carbon nanotube (CNT)-modified surfaces in cyanobacterial biofilm development was assessed over a long-term assay under controlled hydrodynamic conditions. Their impact on the cyanobacterial biofilm architecture was evaluated by novel parameters obtained from three-dimensional (3D) OCT analysis, such as the contour coefficient, total biofilm volume, biovolume, volume of non-connected pores, and the average size of non-connected pores. The results showed that CNTs incorporated into a commercially used epoxy resin (CNT composite) had a higher antifouling effect at the biofilm maturation stage compared to pristine epoxy resin. Along with a delay in biofilm development, a decrease in biofilm wet weight, thickness, and biovolume was also achieved with the CNT composite compared to epoxy resin and glass (control surfaces). Additionally, biofilms developed on the CNT composite were smoother and presented a lower porosity and a strictly packed structure when compared with those formed on the control surfaces. The novel biofilm parameters obtained from 3D OCT imaging are extremely important when evaluating the biofilm architecture and behavior under different scenarios beyond marine applications.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 18