Quantification of alpha-lactalbumin in human milk: Method validation and application

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2007

Autores

Lucia H L M L M Santos (Autor) Outra; Isabel M P L V O Ferreira (Autor) FFUP

Revista

Título: Analytical Biochemistry

Vol. 362

Páginas: 293-295

ISSN: 0003-2697

Editora: Elsevier

Indexação

ISI Web of Knowledge - 6 Citações

Scopus - 5 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-004-B5C

DOI: 10.1016/j.ab.2006.12.002

Abstract (EN): A reversed-phase high-performance liquid chromatography method with ultraviolet detection was optimized and validated for quantification of ¿-lactalbumin in human milk. It was found that the analysis of ¿-lactalbumin can be performed by semiquantitative methods, such as electrophoresis. Samples were skimmed by centrifugation at 5000 rpm, at room temperature, for 5 min, filtered using Whatman No. 42, and diluted 1/5 with deionized water. The external standard method was used to calibrate the system for ¿-lactalbumin quantification. The precision of the method taking into account its repeatability and reproducibility was evaluated. The results show that the method was able to quantify ¿-lactalbumin of human milk with good precision and shorter analysis time.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 3