Codfish authentication by a fast Short Amplicon High Resolution Melting Analysis (SA-HRMA) method

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Tomas, C (Autor) Outra; Ferreira, Isabel (Autor) FFUP; Faria, MA (Autor) Outra

Revista

Título: Food Control

Vol. 71

Páginas: 255-263

ISSN: 0956-7135

Editora: Elsevier

Indexação

ISI Web of Knowledge - 4 Citações

Scopus - 14 Citações

Outras Informações

ID Authenticus: P-00K-N15

DOI: 10.1016/j.foodcont.2016.07.004

Abstract (EN): Atlantic cod (Gadus morhua, L.) is one of the most consumed white fish with high gastronomic relevance in Europe occupying the 8th place in the most captured specie worldwide. G. morhua stocks are depleted configuring a framework favoring the fraudulent substitution of this specie by less valuable ones. The authentication of fish based products at the species level constitutes presently an important issue. Current methods for fish species identification are mainly based on sequencing, a technique that is time and resources consuming boosting the need for the development of more expedite methods. In this work we had developed a one-tube fast High Resolution Melting Analysis (HRMA) method for the identification of G. morhua adulteration with Gadus macrocephalus and Gadus chalcogrammus. HRMA of the ca. 600 bp COI fragment initially assayed proved to be inefficient in species discrimination which lead to the development of a new approach targeting small fragments (ca. 100 bp) containing sequence variations between the three species. Seven small polymorphic regions were identified and specific primer pairs were de novo designed. One region proved to be highly efficient in the species discrimination in HRMA mode. The technique was applied in marketed processed samples showing that 2 out of 33 samples (6%) were mislabeled as G. morhua (identified as G. macrocephalus). PCR and SA-HRMA was performed in fast mode allowing very short analysis time (less than 1 h) enabling therefore the possibility of high sample throughput.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9