Synthesis and analysis of aminochromes by HPLC-photodiode array. Adrenochrome evaluation in rat blood

• Tipo: Artigo em Revista Científica Internacional
• Data: 2003

Autores

Remiao, F (Autor) FFUP; Milhazes, N (Autor) Outra; Borges, F (Autor) FFUP; Carvalho, F (Autor) FFUP; Bastos, ML (Autor) FFUP; Lemos Amado, F (Autor) Outra; Domingues, P (Autor) Outra; Ferrer Correia, A (Autor) Outra

Revista

Título: Biomedical Chromatography

Vol. 17

Páginas: 6-13

ISSN: 0269-3879

Editora: Wiley-Blackwell

Indexação

ISI Web of Knowledge - 26 Citações

Scopus - 29 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química

Outras Informações

ID Authenticus: P-000-J8D

DOI: 10.1002/bmc.203

Abstract (EN): The catecholamine oxidation process induces cardiotoxicity and neurotoxicity. Catecholamines can oxidize to aminochromes through autoxidation or by enzymatic or non-enzymatic catalysis. Although some toxic effects seem to be related to the formation of aminochromes there is still scarce information concerning the identification and evaluation of these compounds in in vivo models. In this study five catecholamines were oxidized to their respective aminochromes: adrenaline/adrenochrome; noradrenaline/noradrenochrome; dopa/dopachrome; dopamine/dopaminochrome; and isoproterenol/isoprenochrome. The evaluation of the catecholamines oxidation profile was performed by HPLC with photodiode array detection and using either enzymatic (tyrosinase) or non-enzymatic [Ag2O, CuSO4, NaIO4 and K3Fe(CN)(6)] catalytic systems. The NaIO4 was found to be the most efficient oxidant of catecholamines. An isocratic reverse-phase HPLC method was developed to analyse each pair of catecholamine-aminochrome. The analytical system was then applied to the detection of adrenochrome in rat blood at 490 nm. Thus, adrenochrome was administered i.p. to rats and its concentration in whole blood was monitored after 5, 15 and 25 min. Blood treatment for adrenochrome evaluation consists of an acidification for protein precipitation followed by a rapid neutralization. The results showed a rapid decrease of adrenochrome concentration in blood after its administration. The adrenochrome present in blood was characterized by UV and tandem mass spectrometry. Copyright (C) 2002 John Wiley Sons, Ltd.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8