Neofunctionalization of the Sec1 alpha 1,2fucosyltransferase Paralogue in Leporids Contributes to Glycan Polymorphism and Resistance to Rabbit Hemorrhagic Disease Virus

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Nystrom, K (Autor) Outra; Abrantes, J (Autor) Outra; Lopes, AM (Autor) Outra; Le Moullac Vaidye, B (Autor) Outra; Marchandeau, S (Autor) Outra; Rocher, J (Autor) Outra; Ruvoen Clouet, N (Autor) Outra; Esteves, PJ (Autor) FCUP; Le Pendu, J (Autor) Outra

Revista

Título: PLoS Pathogens

Vol. 11

ISSN: 1553-7366

Editora: Public Library of Science

Indexação

ISI Web of Knowledge - 7 Citações

Scopus - 9 Citações

Outras Informações

ID Authenticus: P-00G-4SK

DOI: 10.1371/journal.ppat.1004759

Abstract (EN): RHDV (rabbit hemorrhagic disease virus), a virulent calicivirus, causes high mortalities in European rabbit populations (Oryctolagus cuniculus). It uses alpha 1,2fucosylated glycans, histo-blood group antigens (HBGAs), as attachment factors, with their absence or low expression generating resistance to the disease. Synthesis of these glycans requires an alpha 1,2fucosyltransferase. In mammals, there are three closely located alpha 1,2fucosyltransferase genes rSec1, rFut2 and rFut1 that arose through two rounds of duplications. In most mammalian species, Sec1 has clearly become a pseudogene. Yet, in leporids, it does not suffer gross alterations, although we previously observed that rabbit Sec1 variants present either low or no activity. Still, a low activity rSec1 allele correlated with survival to an RHDV outbreak. We now confirm the association between the alpha 1,2fucosyltransferase loci and survival. In addition, we show that rabbits express homogenous rFut1 and rFut2 levels in the small intestine. Comparison of rFut1 and rFut2 activity showed that type 2 A, B and H antigens recognized by RHDV strains were mainly synthesized by rFut1, and all rFut1 variants detected in wild animals were equally active. Interestingly, rSec1 RNA levels were highly variable between individuals and high expression was associated with low binding of RHDV strains to the mucosa. Co-transfection of rFut1 and rSec1 caused a decrease in rFut1-generated RHDV binding sites, indicating that in rabbits, the catalytically inactive rSec1 protein acts as a dominant-negative of rFut1. Consistent with neofunctionalization of Sec1 in leporids, gene conversion analysis showed extensive homogenization between Sec1 and Fut2 in leporids, at variance with its limited degree in other mammals. Gene conversion additionally involving Fut1 was also observed at the C-terminus. Thus, in leporids, unlike in most other mammals where it became extinct, Sec1 evolved a new function with a dominant-negative effect on rFut1, contributing to fucosylated glycan diversity, and allowing herd protection from pathogens such as RHDV.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 25