Molecular cloning and characterization of sea bass (Dicentrarchus labrax L.) CD8 alpha

• Tipo: Artigo em Revista Científica Internacional
• Data: 2006

Autores

Pinto, RD (Autor) Outra; Nascimento, DS (Autor) Outra; do Vale, A (Autor) Outra; dos Santos, NMS (Autor) Outra

Revista

Título: Veterinary Immunology and Immunopathology

Vol. 110

Páginas: 169-177

ISSN: 0165-2427

Editora: Elsevier

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 17 Citações

Outras Informações

ID Authenticus: P-004-MRA

DOI: 10.1016/j.vetimm.2005.11.005

Abstract (EN): In this work, the gene and cDNA of the sea bass CD8 alpha have been isolated and characterized. The coding sequence has an ORF of 666 bp. It retains the Ig motif that interacts with MHC and the two cysteines responsible for an intra-chain disulfide bridge. The hinge region contains the two essential cysteines involved in dimerization. The transmembrane region is well conserved in all analysed sequences. Similar to other teleosts, the cytoplasmic region lacks the consensus p56(1ck) motif common in higher vertebrates. Analysis of the expression pattern using RT-PCR shows the highest expression in the thymus. Like in the human gene, the sea bass CD8 alpha genomic structure is organized into six exons, which roughly correspond to separate functional domains of the protein. Southern blotting shows that CD8 alpha exists as a single copy gene. Together, these results support the concept that the basic structure of CD8a has been maintained through evolution.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9