Thyroxine binding to transthyretin (TTR) variants two variants (TTR Pro 55 and TTR Met 111) with a particularly low binding affinity

• Tipo: Artigo em Revista Científica Internacional
• Data: 1996

Autores

Almeida, MR (Autor) ICBAS; Saraiva, MJ (Autor) ICBAS

Revista

Título: European Journal of Endocrinology

Vol. 135

Páginas: 226-230

ISSN: 0804-4643

Editora: BioScientifica Ltd.

Indexação

ISI Web of Knowledge - 20 Citações

Scopus - 19 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina clínica

Outras Informações

ID Authenticus: P-001-DX1

Abstract (EN): Thyroxine (T-4) binding is a well-characterized function of transthyretin (TTR) and has been used to assess structural alterations of TTR variants. Most TTR variants deposit as amyloid fibrils originating different forms of hereditary amyloidosis. It has been hypothesized that amino acid substitutions in the TTR variants induce structural alterations that may be responsible for the amyloidogenicity of the mutant proteins. To test for structural alterations in TTR, ive studied T-4 binding to TTR variants both in whole serum and in isolated form obtained from heterozygotic carriers of amyloidogenic and nonamyloidogenic variants and compared the binding with the corresponding homozygotic recombinant variants. The results obtained indicated a normal T-4 binding affinity for heterozygotic TTR Ala 491 TTR Leu 68, TTR Ala 71 and TTR Arg 102. Concerning TTR Pro 55 and TTR Met 111, we found a consistent decrease in binding affinity. These results were more pronounced for the equivalent recombinant proteins. Recombinant TTR Pro 55 did not show specific binding to T-4 and recombinant TTR Met 111 presented very low binding affinity. Neither TTR Pro 55 nor TTR Met 111 are localized in the T-4, binding channel, thus structural alterations induced by these mutations should be transmitted to the binding channel interfering with T-4 binding, The results now reported, together with ongoing structural data by X-ray analyses on mutants Pro 55 and Met 111 and future binding studies with other ligands, will contribute to the elucidation of the structure and function of TTR, particularly in thyroid hormone metabolism.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5