Non-genomic Actions of Methylprednisolone Differentially Influence GABA and Glutamate Release From Isolated Nerve Terminals of the Rat Hippocampus

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Neiva, R (Autor) Outra; Caulino Rocha, A (Autor) Outra; Ferreirinha, F (Autor) Outra; Lobo, MG (Autor) ICBAS; Paulo Correia de Sá (Autor) ICBAS

Revista

Título: Frontiers in Molecular Neuroscience

Vol. 13

ISSN: 1662-5099

Editora: Frontiers Media

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 3 Citações

Outras Informações

ID Authenticus: P-00S-MP6

DOI: 10.3389/fnmol.2020.00146

Abstract (EN): Corticosteroids exert a dual role in eukaryotic cells through their action via (1) intracellular receptors (slow genomic responses), or (2) membrane-bound receptors (fast non-genomic responses). Highly vulnerable regions of the brain, like the hippocampus, express high amounts of corticosteroid receptors, yet their actions on ionic currents and neurotransmitters release are still undefined. Here, we investigated the effect of methylprednisolone (MP) on GABA and glutamate (Glu) release from isolated nerve terminals of the rat hippocampus. MP favored both spontaneous and depolarization-evoked [C-14]Glu release from rat hippocampal nerve terminals, without affecting [H-3]GABA outflow. Facilitation of [C-14]Glu release by MP is mediated by a Na+-dependent Ca2+-independent non-genomic mechanism relying on the activation of membrane-bound glucocorticoid (GR) and mineralocorticoid (MR) receptors sensitive to their antagonists mifepristone and spironolactone, respectively. The involvement of Na+-dependent high-affinity EAAT transport reversal was inferred by blockage of MP-induced [C-14]Glu release by DL-TBOA. Depolarization-evoked [H-3]GABA release in the presence of MP was partially attenuated by the selective P2X7 receptor antagonist A-438079, but this compound did not affect the release of [C-14]Glu. Data indicate that MP differentially affects GABA and glutamate release from rat hippocampal nerve terminals via fast non-genomic mechanisms putatively involving the activation of membrane-bound corticosteroid receptors. Facilitation of Glu release strengthen previous assumptions that MP may act as a cognitive enhancer in rats, while crosstalk with ATP-sensitive P2X7 receptors may promote a therapeutically desirable GABAergic inhibitory control during paroxysmal epileptic crisis that might be particularly relevant when extracellular Ca(2+)levels decrease below the threshold required for transmitter release.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 17