Biosensor for label-free DNA quantification based on functionalized LPGs

• Tipo: Artigo em Revista Científica Internacional
• Data: 2016

Autores

Goncalves, HMR (Autor) Outra; Moreira, L (Autor) Outra; Pereira, L (Autor) Outra; Jorge, PAS (Autor) FCUP; Gouveia, C (Autor) Outra; Martins Lopes, P (Autor) Outra; Fernandes, JRA (Autor) Outra

Revista

Título: Biosensors and Bioelectronics

Vol. 84

Páginas: 30-36

ISSN: 0956-5663

Editora: Elsevier

Indexação

ISI Web of Knowledge - 39 Citações

Scopus - 39 Citações

Outras Informações

ID Authenticus: P-00K-M8N

DOI: 10.1016/j.bios.2015.10.001

Abstract (EN): A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62 +/- 2 nM and the limit of quantification was 209 +/- 7 nM. The sample specificity was experimentally demonstrated using DNA targets with different base mismatches relatively to the probe and was found that the system has a single base mismatch selectivity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7