Apresentação Pessoal

Áreas de Interesse

Ciências da Saúde > Ciências Médicas > Metabolómica

Ciências da Saúde > Toxicologia > Metabolómica

Ciências Naturais > Ciências biológicas > Biologia

Ciências Naturais > Química > Química Analítica Instrumental

Ciências Naturais > Química > Produtos alimentares

Domain of specialization

Analytical chemistry and instrumental analysis, mainly Gas and Liquid Chromatography and Mass Spectrometry for application in Biological and Toxicological Research.

 

Current interest of investigation

1. Development of GC-MS methodologies to determine secondary metabolites (low molecular weight compounds) in different biological matrices: in vitro cellular systems, blood, urine, tissues; and food matrices.

2. Metabolomics studies in;

Bladder, Prostate and Renal Cell Carcinoma - studies in in vitro (immortalized culture cells), ex-vivo (tissues) and urines (from cancer patients vs control);

Immortalized culture cells, Primary mouse hepatocytes, and testings in in vivo models for evaluation of the toxicological effects of drugs.

3. Metabolic target studies of “legal” drugs of abuse toxicity by using in vitro and in vivo models.

 

 

Scientific performance

Papers in international scientific periodicals with referees

index h – 50, citations – 7730, in a total 220 publications obtained in March 2025 (scopus).

 

Selected publication works related to Omics

Book Chapter: de Pinho, P.G., Monteiro, M., Carvalho, M., de Lourdes Bastos, M. Potentiality of volatile organic compounds to discriminate patients with cancer by using chemometric tools (2015) Identification and Data Processing Methods in Metabolomics, pp. 166-184. DOI: 10.4155/9781910420287.FSEB2013.14.178

Editor do “special issue” intitulado “Cancer Metabolomics 2018” na revista: Metabolites. ISBN 978-3-03921-345-0 (Pbk); ISBN 978-3-03921-346-7 (PDF) https://doi.org/10.3390/books978-3-03921-346-7 (registering DOI) © 2019 by the authors; CC BY-NC-ND licence Cancer Metabolomics 2018

 

Selected publications in Metabolomics’ area (2019- september 2024):

Carapito A., Roque A.C., Carvalho F., Pinto J., Guedes de Pinho P. (2024). Exploiting Volatile Fingerprints in Bladder Cancer Diagnosis: A Comprehensive Review of Metabolomics and Sensor-Based Approaches Talanta Vol 268, 125296. https://doi.org/10.1016/j.talanta.2023.125296.

Monteiro V., Dias da Silva D., Martins M., Guedes de Pinho P., Pinto J. (2024) Metabolomics perspectives of the ecotoxicological risks of polycyclic aromatic hydrocarbons: A scoping review. Environmental Research 249 (2024.) 118394. doi: 10.1016/j.envres.2024.118394.

Afonso J., Barbosa-Matos C., Silvestre R., Joana Pereira-Vieira Samuel Martins Gonçalves Mendes-Alves C., Parpot P., Pinto J., Carapito A., Guedes de Pinho P., Santos L., Longatto A., Baltazar F. (2024). Cisplatin-resistant urothelial bladder cancer cells undergo metabolic reprogramming beyond the Warburg effect. Cancers 2024, 16, 1418. https://doi.org/10.3390/cancers16071418.

Araújo A.M., Marques S., Guedes de Pinho P., Carmo H., Carvalho F., Silva J. P. (2024). Identification of key neuronal mechanisms triggered by dimethyl fumarate in SH-SY5Y human neuroblastoma cells through a metabolomic approach. Archives Toxicology. 98, 1151–1161. Doi: 10.1007/s00204-024-03683-9.

Oliveira N., Pinho B.R., Pinto J., Guedes de Pinho P., Oliveira J.M.A. (2024). Modelling sporadic als in zebrafish: phenotypic characterisation and rescue effects of edaravone. Free Radical Biology and Medicine, 217, 126-140. https://doi.org/10.1016/j.freeradbiomed.2024.03.016

Amaro F., Carvalho M., Bastos M.L., Guedes de Pinho P., Pinto J. (2024). Metabolomics Reveals Tyrosine Kinase Inhibitor Resistance-Associated Metabolic Events in Human Metastatic Renal Cancer Cells. Int. J. Mol. Sci. 2024, 25, 6328. https://doi.org/10.3390/ijms25126328

Amaro F., Carvalho M., Bastos M.L., Guedes de Pinho P., Pinto J. (2023). Metabolic signatures as prognostic biomarkers for predicting the recurrence of urological cancers. Clinica Chimica Acta. Volume 549, 1 2023, 117553 https://doi.org/10.1016/j.cca.2023.117553.

Dionísio F., Araújo A.M., Duarte-Araújo M., Bastos M.L., Guedes de Pinho P., Carvalho F., Costa V.M. (2022). Cardiotoxicity of cyclophosphamide’s metabolites: an in vitro metabolomics approach in AC16 human cardiomyocytes. Archives of Toxicology, 96(2):653-671. DOI: 10.1007/s00204-021-03204-y.

Amaro F., Carvalho M., Bastos M.L., Guedes de Pinho P., Pinto J. (2022).  Pharmacometabolomics Applied to Personalized Medicine in Urological Cancers. Pharmaceuticals 2022, 15(3), 295; https://doi.org/10.3390/ph15030295.

Soares R., Manguinhas R., Costa J. G., Saraiva N., Gil N., Rosell R., Camões S.P., Batinic-Haberle I., Spasojevic I., Castro M., Miranda J.P., Amaro F., Pinto J., Fernandes A.S., Guedes de Pinho P., Oliveira N. G. (2022). MnTnHex-2-PyP5+ Displays Anticancer Properties and Enhances Cisplatin effects in Non-Small Cell Lung Cancer Cells. Antioxidants 11(11), 2198; https://doi.org/10.3390/antiox11112198.

Pinto J., Amaro F., Lima A.R., Carvalho-Maia C., Jerónimo C., Henrique R., Bastos M.L., Carvalho M., Guedes de Pinho P. (2021). Urinary volatilomics unveils a candidate biomarker panel for non-invasive detection of clear cell renal cell carcinoma. J. Proteome Research 20(6):3068-3077. acs.jproteome.0c00936. ” Editor’s choice: Graphical Abstract selected for a Supplementary Cover Issue”.

Lima A.R, Pinto J., Amaro F., Bastos M.L., Carvalho M., Guedes de Pinho P. (2021). Advances and Perspectives in Prostate Cancer Biomarker Discovery in the Last 5 Years through Tissue and Urine Metabolomics. Metabolites, 11(3), 181; doi.org/10.3390/metabo11030181. Hot Topic Papers Published in 2021 in the “Cell Metabolism” Section e and choice of Metabolites Editor.

Pinto J., Carapito A., Amaro F., Lima A.R., Carvalho-Maia C., Jerónimo C., Henrique R., Bastos M.L., Guedes de Pinho P. (2021) Impact of Bladder Cancer on the Urinary Volatilome. Metabolites, 11(4), 199; doi.org/10.3390/metabo11040199. Hot Topic Papers Published in 2021 in the “Cell Metabolism”

Lima A.R, Carvalho M., Aveiro S.S., Melo T., Domingues M.R., Macedo-Silva C., Coimbra N., Jerónimo C., Henrique R., Bastos M.L., Guedes de Pinho P., Pinto J. (2021) Comprehensive metabolomics and lipidomics profiling of prostate cancer tissue reveals metabolic dysregulations associated with disease development. J. Proteome Research https://doi.org/10.1021/acs.jproteome.1c00754

Araújo A.M., Bastos M.L., Carvalho F., Guedes de Pinho P., Carvalho M. (2020) Effect of temperature on 3,4-Methylenedioxypyrovalerone (MDPV)-induced metabolome disruption in primary mouse hepatic cells Toxicology, https://doi.org/10.1016/j.tox.2020.152503

Amaro F, Pinto J., Rocha S., Araújo A.M., Miranda-Gonçalves V., Jerónimo C., Henrique R., Bastos M.L., Carvalho M., Guedes de Pinho P. Volatilomics Reveals Potential Biomarkers for Identification of Renal Cell Carcinoma: An In Vitro Approach. Metabolites 2020, 10, 174; doi:10.3390/metabo10050174.

Lima A.R., Pinto J., Barros-Silva D., Jerónimo C., Henrique R., Bastos M.L., Carvalho M., Guedes de Pinho P. (2020). Combined use of 1H NMR and GC-MS analytical platforms for metabolomic profiling of urine from PCa patients. Metabolomics, 16(6):70. doi: 10.1007/s11306-020-01691-1.

Rodrigues, D.; Pinto, J.; Araújo, A.M.; Jerónimo, C.; Henrique, R.; Bastos, M.L Guedes de Pinho, P., Carvalho M.  GC-MS Metabolomics Reveals Distinct Profiles of Low- and High-Grade Bladder Cancer Cultured Cells (2019). Metabolites, 9, 18, DOI: 10.3390/metabo9010018.

Araújo A.M., Carvalho M., Bastos M.L., Guedes de Pinho P. Metabolic signature of methylone in primary mouse hepatocytes, at subtoxic concentrations (2019) Archive Toxicology 93, 3277–3290.  DOI: 10.1007/s00204-019-02566-8.

Lima A.R., Pinto J., Azevedo A.I., Barros-Silva D., Jerónimo C., Henrique R., Bastos M.L., Guedes de Pinho P., Carvalho M. (2019) Identification of a biomarker panel for improvement of prostate cancer diagnosis by volatile metabolic profiling of urine. British J Cancer 121, 857–868.

Araújo A.M., Enea M., Carvalho F. Bastos M.L., Carvalho M., Guedes de Pinho P. (2019) Hepatic metabolic derangements triggered by hyperthermia: an in vitro metabolomic study Metabolites. 9 (10), 228.

Enea M., Araújo A.M., Peixoto de Almeida M., Soares M.E., Gonçalves-Monteiro S., Guedes de Pinho P., Pereira E., Bastos M.L., Carmo H. (2019) A preliminary metabolomic approach for the in vivo study of gold nanospheres and nanostars after a single-dose intravenous administration to Wistar rats. Nanomaterials, 9, 1606, 1-23. DOI: 10.339/nano9111606.