Bacteriology

Code:

MI073122

Acronym:

BACTER

Keywords
Classification	Keyword
OFICIAL	Health Sciences

Instance: 2019/2020 - 1S

Active?	Yes
Responsible unit:	Microbiology Laboratory
Course/CS Responsible:	MSc in Pharmaceutical Sciences

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MICF	185	Official Curriculum	3	-	6	52	162

Teaching language

Suitable for English-speaking students

Objectives

Recognition of the importance of bacterial flora to human health and acquisition of knowledge on relevant bacterial features of infectious agents in order to understand the conditions allowing their growth in different niches, control, identification and pathogenicity. Omics technologies interest and their suitability for biological systems research and bacterial characterization will be also studied. The antimicrobial agents of prophylactic and therapeutic use as well as the main mechanisms of bacterial resistance to antimicrobials will be also studied. 

Learning outcomes and competences

• Ability to prevent and contain bacterial infectious diseases


• Comprehension of the relevance of microbiota in health and disease, acting to preserve or recover its equilibrium


• Comprehension of the genetic and environmental drivers of bacterial diversity and pathogenicity


• Comprehension of the potential and drawbacks of different methodologies for bacterial characterization, research and laboratory diagnostic purposes.


• -Ability to implement different methodologies to detect and identify bacteria of interest in human clinical context, food sector, pharmaceutical industry and investigation;


• Ability to interpret bacterial information obtained by genomic/genotypic characterization either in the context of antimicrobial resistance studies or epidemiologic purposes.


• Ability to implement methodologies to predict the therapeutic efficacy of antimicrobials and its interpretation in clinical and microbiologic context (ECOFFs).


• Ability to detect resistance to antimicrobials by different techniques

Working method

Presencial

Program

• 
  

  Theoretical Component:

  
  
  
  
  • Bacterial diversity: key features and methodologies for their study. Core and accessory genomes; 
  
  
  • Human Microbiome and current advances on disease elucidation; Normal flora and of colonization. Infection; 
  
  
  • Antibiotics and mechanisms of resistance of relevance in particular bacteria (e.g: MRSA, ESBL and carbapenemase Gram negative producers);
  
  
  • Study of the main bacterial ethiologic agents of human infections (phylogeny, morphological and physiological characteristics, virulence factors, clinical presentations, prevention, laboratorial diagnostic and therapy)
  
  
  
  

  Practical 

Component:

  
  

  1- Culturomic and metagenomic for study of human microbiota. The examples of female urinary and hands microbiota; 2- Laboratorial diagnostic of bacterial infections of urinary and respiratory tract; 3- Identification by classical methods of bacterial human pathogenics; 4- Serodiagnostic. Serologic classification; 5- Evaluation of the efficacy of antimicrobials: concepts and methods (diffusion method with discs; Minimum Inhibitory concentration determination); 6- Molecular methods applied to typing (MALDI-TOF MS; WGS).

  
  

Mandatory literature

João Carlos Sousa, Elisabete Machado, Carla Novais, Luísa Peixe, José Amorim & Nuno Monteiro; Antibióticos. Vol. I , Edições Universidade Fernando Pessoa, 2016
Hauser Alan R.; Antibiotic Basics for Clinicians, Loppincott Williams & Wilkins, 2013
Patricia Tille; Bailey & Scott's Diagnostic Microbiology, Mosby. Elsevier, 2014. ISBN: 978-0-323-08330-0

Complementary Bibliography

James H. Jorgensen1, Michael A. Pfaller2, Karen C. Carroll3, Guido Funke4, Marie Louise Landry5, Sandra S. Richter6, David W. Warnock; Manual of Clinical Microbiology, Eleventh Edition, ASM, 2015. ISBN: 9781555817374

Teaching methods and learning activities

a)Theoretical classes: 2 horas /week. Expositive method will be used in the theoretical component, supported on power point to transmit fundamental aspects of each topic. Further individual study on the topic presented will be encouraged (book chapters, scientific papers, videos or webpages)

b)Practical classes: 2 hours/week.

The laboratory work will be developed in group or individually.  The objectives of the laboratorial works are the acquisition of skills to execute different important methodologies for diagnostic of a bacterial issue on different matrices (e.g. biologic samples, food), track bacteria and determine the suitable antibiotics for treatment of human infections. Comprehension of related concepts and the acquisition of competencies on those aspects in the remit of pharmaceutical activity will be persued. Additionally, consolidation of concepts mentioned in the theoretical classes will be also envisaged;

2h/week will be reserve for students attendance. Students should send an email to the responsible teacher requesting an appointment.

Evaluation Type

Distributed evaluation with final exam

Assessment Components

designation	Weight (%)
Exame	80,00
Teste	20,00
Total:	100,00

Amount of time allocated to each course unit

designation	Time (hours)
Frequência das aulas	0,00
Total:	0,00

Eligibility for exams

According to the evaluation rules of FFUP

Calculation formula of final grade

Final mark: 80% of the exam of the theoretical part + 20% of practical mark

Concerning the evaluation of the practical part:

Laboratorial exam will occur during 16-20 december 2019. In case of failure or absence to this exam, the students will have another opportunity in the Normal Period of Examens (Época Normal de exames).
To improve their mark repetition of the exam could be performed in Normal Period of Examens (Época Normal de exames)

For approval, the marks in each of the theoretical and practical exams should be equal or higher than 9,5 (scale of 20 values).

Examinations or Special Assignments

The approval on a final exam for evaluation of practical classes component is required for student admission to the theoretical exam. 

Students that are not obliged to assist laboratory classes have to perform a final exam for evaluation of practical classes component. 

Special assessment (TE, DA, ...)

According to the evaluation rules of FFUP

Classification improvement

Exams for improving theoretical and practical results could be requested by the students

Observations

It is recommended the previous attendance of the following courses: General microbiology, Celular Biology, Imunology and Genetics.