Cellular Biology
Keywords |
Classification |
Keyword |
OFICIAL |
Natural Sciences |
Instance: 2012/2013 - 1S
Cycles of Study/Courses
Acronym |
No. of Students |
Study Plan |
Curricular Years |
Credits UCN |
Credits ECTS |
Contact hours |
Total Time |
MICF |
264 |
Official Curriculum |
1 |
- |
6,5 |
65 |
175,5 |
Teaching language
Portuguese
Objectives
It is intended that students obtain: i) knowledge of the basic principles of biochemistry and main experimental methodologies for the study of cell ii) understanding the properties common to all eukaryotic cells: expression and transmission of genetic information, the internal organization of cells . These skills are fundamental to the subjects that are taught throughout the course.
Program
The evolution of the cell. Understanding chemical components of cells and cellular energy. Methods for studying cell: microscopy, cell fractionation and analysis of their molecules. Basic genetic mechanisms: transcription, mRNA processing, translation, and DNA replication. Mechanisms of DNA repair. Composition of biomembranes and membrane transport. Internal organization of the cells: the cytosol, structure and function of various organelles (peroxisomes, endoplasmic reticulum, Golgi apparatus, lysosomes, mitochondria). Nucleus and nucleolus. Cytoskeleton. Intercellular junctions, cell adhesion and extracellular matrix. Cell signaling. Cell death and cancer. Cell cycle: interphase and mitosis. Meiosis.
Laboratory program
Cytological Techniques: i) Wet mounts ii) Final preparations without inclusion; iii) Final preparations with inclusion Measurement of cells, staining by immunohistochemistry and in situ hybridization. Separation of cells. Photographs of electron microscopy. Research literature on various topics
Mandatory literature
Harvey Lodish, Arnold Berk, Chris Kaiser, Monty Krieger, Matthew Scott, Anthony Bretscher, Hidle Ploegh, Paul Matsudaira; Molecular Cell Biology, Scientific American Books, W.H. Freeman Company, New York, 2007. ISBN: 13-978-0-7167-7601-7
Alberts Bruce;
Molecular biology of the cell. ISBN: 0-8153-4106-7
Complementary Bibliography
Carlos Azevedo; Biologia Celular e Molecular, ., Lidel, edições técnicas, 2005
J.K. Presnell, M.P. Schreibman; Humason´s – Animal Tissue Techniques, the Johns Hopkins University Press, Baltimore & London, 1997
Teaching methods and learning activities
a) Lectures (3 hours / week): master classes illustrated using the data show and videos. b) The laboratory work (2 hours / week) are performed individually and according to the schedule previously established. In the first lecture is provided all the fundamentals and protocols of work performed in the laboratory classes c) In laboratory classes students will report to the corresponding literature search conducted on topics proposed by the Lecturers d) The Lecturer in charge is available in the hours announced early in the semester and during exams by appointment at any other time.
Evaluation Type
Distributed evaluation with final exam
Assessment Components
Description |
Type |
Time (hours) |
Weight (%) |
End date |
Attendance (estimated) |
Participação presencial |
65,00 |
|
|
|
Total: |
- |
0,00 |
|
Eligibility for exams
-The assistance of students to the laboratory classes is mandatory, being considered infrequently students whose attendance is less than 75% of classes planned
- The attendance of students at lectures is not mandatory.
Calculation formula of final grade
The final grade is the sum of the final exam (90%) and distributed evaluation (10%). In the final exam the theoretical part corresponds to 80% and the practical to 10%. The distributed evaluation is composed by i) reports in laboratory classes ii) examination held in the last laboratory class. Assessment is expressed on a scale of 0 to 20. Students rated below 9.5 are considered disapproved. There are no oral exams.
Examinations or Special Assignments
Students who are exempted by law from the presence of laboratory classes are required to perform a exam laboratory. The approval of this laboratory test is a prerequisite for admission to the student's final exam written test.
Special assessment (TE, DA, ...)
Students may apply for grade improvement only once per subject, one of the two times, normal or appeal, immediately subsequent to that they received approval.
The final classification will be the highest of those obtained in the two tests performed.
Classification improvement
Students may apply for grade improvement only once per subject, one of the two times, normal or appeal, immediately subsequent to that they received approval.
The final classification will be the highest of those obtained in the two tests performed.
Observations
Students should have a basic knowledge of chemistry and biology taught in secondary schools.
Teaching Language: Portuguese and eventually English.