Go to:
Logótipo
Você está em: Start > Publications > View > Separation of the heme protein cytochrome C using a 3D structured graphene oxide bionanocomposite as an adsorbent
Publication

Separation of the heme protein cytochrome C using a 3D structured graphene oxide bionanocomposite as an adsorbent

Title
Separation of the heme protein cytochrome C using a 3D structured graphene oxide bionanocomposite as an adsorbent
Type
Article in International Scientific Journal
Year
2024
Authors
de Araujo, CMB
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Rios, AG
(Author)
FEUP
View Personal Page You do not have permissions to view the institutional email. Search for Participant Publications View Authenticus page View ORCID page
Ghislandi, MG
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Alexandre Ferreira
(Author)
FEUP
View Personal Page You do not have permissions to view the institutional email. Search for Participant Publications View Authenticus page View ORCID page
Sobrinho, MAD
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Rodrigues, AE
(Author)
Other
The person does not belong to the institution. The person does not belong to the institution. The person does not belong to the institution. Without AUTHENTICUS Without ORCID
Journal
Title: Soft MatterImported from Authenticus Search for Journal Publications
Vol. 20
Pages: 1475-1485
ISSN: 1744-683X
Indexing
Publicação em ISI Web of Knowledge ISI Web of Knowledge - 0 Citations
Publicação em Scopus Scopus - 0 Citations
Other information
Authenticus ID: P-00Z-XTD
Abstract (EN): Proteins are of great importance for medicine and the pharmaceutical and food industries. However, proteins need to be purified prior to their application. This work investigated the application of a hydrogel bionanocomposite based on agar and graphene oxide (GO) for capturing cytochrome C (Cyto C) heme protein by adsorption from aqueous solutions with other proteins. Although applications of GO-based materials in adsorption are widely studied, the focus on semi-continuous processes remains limited. Adsorption experiments were carried out in batch and fixed bed columns. The effect of pH and ionic strength on adsorption was investigated, and there is evidence that electrostatic interactions between Cyto C and the nanocomposite were favoured at pH = 7; the adsorption capacity decreased as NaCl and KCl concentrations increased, ascribed to the weak electrostatic interaction between the protein and GO active sites in the bionanocomposite. All adsorption isotherm models (Langmuir, Freundlich, Sips) used gave suitable adjustments to the equilibrium experimental data and the kinetic models applied. The maximum adsorption capacity predicted by the Langmuir isotherm was similar to 400 mg(CytoC) g(adsorbent,dry)(-1), and the adsorption thermodynamics indicated a physisorption process. Tests were performed to evaluate the co-adsorption in batch, and the composite was effective in adsorbing Cyto C in solution with bovine serum albumin (BSA) and L-phenylalanine. Fixed bed tests were performed, and although protein adsorption onto nanoparticles can be challenging, the Cyto C adsorbed could be successfully recovered after desorption. Overall, the GO-based hydrogel was an effective method for cytochrome C adsorption, exhibiting a notorious potential for applications in protein separation processes.
Language: English
Type (Professor's evaluation): Scientific
No. of pages: 11
Documents
We could not find any documents associated to the publication.
Related Publications

Of the same journal

When is a surface foam-phobic or foam-philic? (2018)
Article in International Scientific Journal
Teixeira, MAC; Arscott, S; Cox, SJ; Teixeira, PIC
The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors (2013)
Article in International Scientific Journal
Diana Paiva; Albertoo Martín-Molina; Isabel Cardoso; Manuel Quesada-Pérez; Maria do Carmo Pereira; Sandra Rocha
Stabilizing effect of shear thinning on the onset of purely elastic instabilities in serpentine microflows (2016)
Article in International Scientific Journal
Laura Casanellas; Manuel A. Alves; Robert J. Poole; Sandra Lerouge; Anke Lindner
Shaping the molecular assemblies of native and alkali-modified agars in dilute and concentrated aqueous media via microwave-assisted extraction (2013)
Article in International Scientific Journal
Ana M. M. Sousa; João Borges; Fernando Silva; Ana M. Ramos; Eurico J. Cabrita; Maria Pilar Gonçalves

See all (28)

Recommend this page Top
Copyright 1996-2025 © Faculdade de Farmácia da Universidade do Porto  I Terms and Conditions  I Acessibility  I Index A-Z  I Guest Book
Page created on: 2025-06-24 at 02:07:00 | Acceptable Use Policy | Data Protection Policy | Complaint Portal