New insights into a bacterial metabolic and detoxifying association responsible for the mineralization of the thiocarbamate herbicide molinate

• Type: Article in International Scientific Journal
• Date: 2008

Authors

Luisa Barreiros (Author) FEUP; Ana Fernandes (Author) Other; Antonio Silva S Ferreira (Author) Other; Helena Pereira (Author) Other; Margarida M. S. M. Bastos (Author) FEUP; Celia M Manaia (Author) Other; Olga C Nunes (Author) FEUP

Journal

Title: Microbiology

Vol. 154 No. 4

Pages: 1038-1046

ISSN: 1350-0872

Publisher: Microbiological Society

Indexing

ISI Web of Knowledge - 19 Citations

Scopus - 23 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

CORDIS: Technological sciences > Engineering > Chemical engineering ; Natural sciences > Biological sciences > Biology > Microbiology

Other information

Authenticus ID: P-004-0N8

DOI: 10.1099/mic.0.2007/015297-0

Abstract (EN): A novel pathway of molinate mineralization promoted by a defined mixed culture composed of five bacteria (named ON1 to ON5) was proposed previously. Evidence was obtained of a metabolic association between Gulosibacter molinativorax ON4(T), capable of molinate breakdown, and the remaining bacteria. In the present study, the role of each isolate in that metabolic association was further explored and the possible synergistic effect of all the bacterial isolates for the stability of the mixed culture is discussed. The cleavage of the molinate thioester bond, whether occurring under aerobic or anaerobic conditions, releases ethanethiol (S-ethyl moiety) and an azepane moiety derivative, identified as azepane-1-carboxylic acid. This azepane moiety is degraded, in the presence of oxygen, by Pseudomonas strains ON1 and ON3 and G. T T molinativorax ON4. Ethanethiol, which inhibits G. molinativorax ON4, is consumed by Pseudomonas strain ON1 and Stenotrophomonas maltophilia ON2. Although a two-member mixed culture of G. molinativorax ON4 T and Pseudomonas strain ON1 was able to promote the aerobic mineralization of molinate, after 20 successive transfers of the five-member mixed culture in mineral medium with molinate, none of these isolates were lost. The results obtained indicate that the whole mixed culture may have a higher fitness than the two-member culture, even when the basic degradative and cross-protection functions are assured.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9