Influence of high-fat diet on bone tissue: An experimental study in growing rats

• Type: Article in International Scientific Journal
• Date: 2017-12

Authors

G. Rezende Yanagihara (Author) Other; R. Carminati Shimano (Author) Other; J. Atsuko Tida (Author) Other; J. Suzuki Yamanaka (Author) Other; S. Yasuko Fukada (Author) Other; J.P. Madergan Issa (Author) Other; A.C. Shimano (Author) Other; João Manuel R. S. Tavares (Author) FEUP

Journal

Title: Journal of Nutrition, Health and Aging

Vol. 21

Pages: 1337-1343

ISSN: 1279-7707

Publisher: Springer Nature

Indexing

ISI Web of Knowledge - 2 Citations

ISI Web of Science

Scopus - 6 Citations

Scientific classification

CORDIS: Health sciences

FOS: Engineering and technology

Other information

Authenticus ID: P-00M-CKF

DOI: 10.1007/s12603-017-0871-x

Abstract (EN): The relationship between obesity and bone tissue remains contradictory, especially when the effect of high-fat diet is assessed in experimental models. The aim of this study was to evaluate the effects of high-fat diet on bone metabolism of growing rats. Twenty weaned female Wistar rats were equally divided into two groups: SD (standard diet) and HFD (high-fat diet with 60 % of energy as fat). After five weeks of the two diets, the rats were euthanized, and the liver, blood and bones extracted. The liver was analysed for malondialdehyde (MDA) and reduced glutathione (GSH) concentrations. Blood was analysed by the ELISA method for osteoprotegerin (OPG) and tumour necrosis factor ligand superfamily member 11 (TNFSF11/RANKL). The bone tissue was analysed by dual-energy X-ray absorptiometry (DXA), mechanical tests, computed microtomography, histological quantitative analysis and scanning electron microscopy. The gene expressions of PPAR-gamma Runx-2, RANKL and Cathepsin-K were also evaluated. HFD caused an increase in the MDA concentration, indicating oxidative stress. It also increased the expression of PPAR-gamma, which is the gene that is related to adipocyte differentiation. There was an increase in BMD of the tibia of animals fed with the HFD, but other microstructural and mechanical properties were maintained unaltered. In addition, there were no changes in the gene expressions related to the differentiation of osteoblasts and osteoclasts, as well as no changes to the biochemical markers of bone formation and bone resorption. Liver and gene parameters are changed in response to the HFD. However, although there was an increase in BMD, the microstructure and function of the bone did not change after a 5-week HFD.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7