Upregulation of bone cell differentiation through immobilization within a synthetic extracellular matrix

• Tipo: Artigo em Revista Científica Internacional
• Data: 2007

Autores

Marta B Evangelista (Autor) Outra; Susan X Hsiong (Autor) Outra; Rui Fernandes (Autor) Outra; Paula Sampaio (Autor) Outra; Hyun Joon Kong (Autor) Outra; Cristina C Barrias (Autor) Outra; Roberto Salema (Autor) Outra; Mario A Barbosa (Autor) FEUP; David J Mooney (Autor) Outra; Pedro L Granja (Autor) FEUP

Revista

Título: Biomaterials

Vol. 28

Páginas: 3644-3655

ISSN: 0142-9612

Editora: Elsevier

Indexação

ISI Web of Knowledge - 82 Citações

Scopus - 84 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Biotecnologia ambiental

CORDIS: Ciências Tecnológicas > Engenharia > Engenharia de biomateriais

Outras Informações

ID Authenticus: P-004-7M7

DOI: 10.1016/j.biomaterials.2007.04.028

Abstract (EN): There is a need for new therapeutic strategies to treat bone defects caused by trauma, disease or tissue loss. Injectable systems for cell transplantation have the advantage of allowing the use of minimally invasive surgical procedures, and thus for less discomfort to patients. In the present study, it is hypothesized that Arg-Gly-Asp (RGD)-coupled in a binary (low and high molecular weight) injectable alginate composition is able to influence bone cell differentiation in a three-dimensional (3D) structure. Viability, metabolic activity, cytoskeleton organization, ultrastructure and differentiation (alkaline phosphatase (ALP), von Kossa, alizarin red stainings and osteocalcin quantification) of immobilized cells were assessed. Cells within RGD-modified alginate microspheres were able to establish more interactions with the synthetic extracellular matrix as visualized by confocal laser scanning microscope and transmission electron microscopy imaging, and presented a much higher level of differentiation (more intense ALP and mineralization stainings and higher levels of osteocalcin secretion) when compared to cells immobilized within unmodified alginate microspheres. These findings demonstrate that peptides covalently coupled to alginate were efficient in influencing cell behavior within this 3D system, and may provide adequate preparation of osteoblasts for cell transplantation.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12